FastQCFastQC Report
Mon 6 Mar 2017
G151-M12-Index-23_GAGTGG-_BCALRAANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M12-Index-23_GAGTGG-_BCALRAANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26557590
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA12750.018.80540843
CTCGTAT13100.018.63880744
GTCACGA14500.017.29496629
CGTCTGA17050.015.09774916
CACGAGT16600.014.84089431
TATCTCG16050.014.80132141
ACGAGTG16350.014.7988932
CGAGTGG17350.013.94587533
TCACGAG19400.013.26674930
ATCTCGT18250.013.25798442
AGTCACG20950.012.60058428
ACACGTC21050.012.33361513
ACGTCTG22550.011.6103115
ATCGGAA26150.09.3377332
GATCGGA28000.09.1990641
CGGAAGA32250.08.3890744
TCGGAAG31500.08.3090663
CACGTCT35550.07.674093214
GCACACG38600.07.466929411
CACACGT38550.07.13417412
Produced by FastQC (version 0.11.2)