FastQCFastQC Report
Mon 6 Mar 2017
G151-M8-Index-19_GTGAAA-_BCALRAANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M8-Index-19_GTGAAA-_BCALRAANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25619778
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG8000.034.10716612
GCGTCGT8600.031.7276611
AGCGTCG8700.030.6041910
GTCGCCG6400.030.59820744
GTCGTGT13950.020.50610213
GAGCGTC14000.019.0183189
TGGTCGC11050.017.91843442
TCGTGTA16850.017.10745214
CGTGTAG17550.016.0485515
GGTCGCC12700.015.76524543
GATCTCG14600.015.36763334
GTGGTCG13350.015.32364841
GATCGGA18350.014.5079921
AGAGCGT18700.014.4734898
ATCGGAA19650.013.884082
AAGAGCG20650.013.2129927
TCGGTGG19250.013.02634938
TCGGAAG24250.011.8856423
TCTCGGT20350.011.34954936
ATCTCGG20500.010.94471135
Produced by FastQC (version 0.11.2)