FastQCFastQC Report
Mon 6 Mar 2017
G151-M6-Index-16_CCGTCC-_BCALRAANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M6-Index-16_CCGTCC-_BCALRAANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25801346
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG12650.021.38634743
CTCGTAT13350.020.92573442
TCTCGTA12900.020.1208541
CGTATGC14200.019.20692844
ACCCGTC14200.019.05650332
CCGTCCA16050.017.27167934
CGTCCAT16100.016.67169435
TCACCCG17050.015.99957930
CACCCGT17400.015.80473531
CGTCTGA17450.015.75721816
ATCTCGT18500.014.74318740
GATCGGA19400.014.6354631
ATCGGAA20000.014.0770382
ACACGTC20150.013.86580313
ACGTCTG20900.013.156095515
CATCTCG21500.012.27734339
CCCGTCC25550.011.02187833
TCGGAAG25950.011.0164633
CGGAAGA27600.010.7567294
CACGTCT34250.08.60657314
Produced by FastQC (version 0.11.2)