FastQCFastQC Report
Mon 6 Mar 2017
G151-M5-Index-15_ATGTCA-_BCALRAANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M5-Index-15_ATGTCA-_BCALRAANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24073974
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG8800.033.5053112
AGCGTCG9200.031.57008610
GCGTCGT9450.031.2007111
GTCGCCG7500.029.04375544
GATCGGA14000.021.3718831
GTCGTGT14250.020.99951213
GAGCGTC14350.020.3933479
TGGTCGC11050.019.31254642
CGTGTAG16200.018.47163815
TCGTGTA16500.018.40245614
ATCGGAA16350.018.0306262
GATCTCG14250.017.44388434
GGTCGCC12650.017.2185843
GTGGTCG13650.016.59925841
AGAGCGT18150.016.3659558
TCGGAAG22400.014.044653
AAGAGCG21000.014.0399987
ATCTCGG19100.013.59027435
TCGGTGG19550.013.16479838
CGGAAGA24150.012.9362014
Produced by FastQC (version 0.11.2)