FastQCFastQC Report
Mon 6 Mar 2017
G151-M5-Index-15_ATGTCA-_BCALRAANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M5-Index-15_ATGTCA-_BCALRAANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24073974
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA13100.021.65902743
CTCGTAT13950.021.60074444
GATCGGA13950.021.1425361
ATCGGAA16500.017.9960672
AATCTCG16600.017.35876841
CGTCTGA18250.017.35615316
ACGTCTG19400.015.64697515
ACACGTC20200.015.57327913
ATCTCGT19750.015.14693742
TCGGAAG21000.014.450863
CGGAAGA24400.012.5278874
CACGTCT32450.09.82942214
GCACACG35400.08.94855411
CACACGT37550.08.78769412
ACATGTC67500.05.475678432
GAACTCC72150.05.064231421
Produced by FastQC (version 0.11.2)