FastQCFastQC Report
Mon 6 Mar 2017
G151-M4-Index-11_GGCTAC-_BCALRAANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M4-Index-11_GGCTAC-_BCALRAANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27126881
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG7850.029.15179812
GTCGCCG6600.026.00362844
GCGTCGT9000.025.42684611
AGCGTCG9300.024.60653510
TGGTCGC11150.015.78506342
GTCGTGT15250.015.14993313
GAGCGTC15600.015.0924059
TCGTGTA16550.014.358603514
CGTGTAG16450.014.17860915
GGTCGCC12500.013.90532443
GATCTCG15100.013.25658334
GATCGGA17800.012.9778791
GTGGTCG14350.012.57002941
ATCGGAA19350.012.3928252
AGAGCGT20600.011.6427868
AAGAGCG21800.010.7999717
ATCTCGG19250.010.3986535
TCGGAAG24100.010.31528953
TCTCGGT18400.010.16172536
TCGGTGG21250.09.73046738
Produced by FastQC (version 0.11.2)