FastQCFastQC Report
Mon 6 Mar 2017
G151-M2-Index-9_GATCAG-_BCALRAANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M2-Index-9_GATCAG-_BCALRAANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25159044
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG9600.031.62963512
GCGTCGT9850.031.05023811
AGCGTCG10000.030.14435810
GTCGCCG8050.029.51862144
GATCGGA14600.021.2471961
GTCGTGT15800.019.6353613
TGGTCGC12100.019.2732942
TCGTGTA16050.019.05506914
GAGCGTC16200.019.0148689
CGTGTAG17900.017.57771515
GATCTCG14750.017.29982834
ATCGGAA18400.017.0977822
GGTCGCC14500.016.5390443
GTGGTCG14700.016.46116841
AGAGCGT20150.015.7241398
AAGAGCG22750.014.1204157
TCGGTGG20450.013.12322638
ATCTCGG20950.012.49507135
CGGTGGT20750.012.40343139
TCTCGGT21250.012.3186736
Produced by FastQC (version 0.11.2)