FastQCFastQC Report
Mon 6 Mar 2017
G151-M2-Index-9_GATCAG-_BCALRAANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M2-Index-9_GATCAG-_BCALRAANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25159044
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG14200.022.46044743
CTCGTAT14750.021.47501242
CGATCAG14500.021.24255433
TCTCGTA14500.021.23871241
CGTATGC15300.020.70188144
GTCACGA15150.020.18340729
GATCTCG16300.019.70371239
GATCGGA15800.019.643841
ACGATCA16500.018.66732832
CACGATC16700.018.4437331
CGTCTGA18200.017.76590316
TCACGAT18500.017.00526430
ATCGGAA18650.016.74832
ACGTCTG20250.016.07593715
ACACGTC21400.015.111032513
ATCTCGT21600.014.56263740
AGTCACG23800.013.58736728
TCGGAAG24100.013.0483863
CGGAAGA25900.012.4817174
CACGTCT32950.010.21411414
Produced by FastQC (version 0.11.2)