FastQCFastQC Report
Mon 6 Mar 2017
G151-M11-Index-22_CGTACG-_BCALRAANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M11-Index-22_CGTACG-_BCALRAANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28567705
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG6500.029.44173633
CGTACGT6550.028.88106234
ACCGTAC10200.019.19323232
GTACGTA10700.018.09087235
CTCGTAT12150.016.65625844
CGTAATC10800.016.29350938
TCTCGTA11650.015.86058743
GATCGGA12750.015.5390791
CACCGTA14050.014.40338231
TACGTAA14600.013.8609136
ATCGGAA15600.012.9734582
AATCTCG15250.012.40458941
GTCACCG16800.012.04652929
CGTCTGA17150.011.80247916
ACGTAAT16650.011.4935937
ATCTCGT18650.010.61488142
ACACGTC21250.09.93978613
TCACCGT21500.09.515367530
TCGGAAG22250.09.4898973
ACGTCTG23050.08.68592715
Produced by FastQC (version 0.11.2)