FastQCFastQC Report
Mon 6 Mar 2017
G151-M10-Index-21_GTTTCG-_BCALRAANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M10-Index-21_GTTTCG-_BCALRAANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24402850
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG4150.027.032233
GATCGGA9700.013.1642371
GTTTCGG9900.012.66466334
CTCGTAT10050.012.0379744
TTTCGGA10350.011.68904335
CGGAATC10150.011.05218838
ATCGGAA11150.011.0491182
CGTCTGA12500.010.20862316
TCGGAAT10350.010.20117937
TCTCGTA11050.010.15232343
TTCGGAA13750.09.11855636
ACACGTC14950.08.38876413
TCGGAAG17150.08.0798073
AATCTCG13250.07.96843641
ACGTCTG17100.07.848335315
GTCACGT17250.07.26890629
ATCTCGT15750.07.262163642
AGTCACG18000.07.21053128
ACGTTTC20600.06.406844632
CGGAAGA20450.06.3460664
Produced by FastQC (version 0.11.2)