FastQCFastQC Report
Mon 6 Mar 2017
G151-M1-Index-8_ACTTGA-_BCALRAANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M1-Index-8_ACTTGA-_BCALRAANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27150749
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG10250.030.91257510
CGTCGTG10300.029.4807412
GCGTCGT10750.028.86072211
GTCGCCG8450.027.8610344
GAGCGTC14450.021.6228549
GTCGTGT16400.019.1858713
TGGTCGC12700.018.70908542
CGTGTAG17150.018.60333415
TCGTGTA17150.018.21820314
GTGGTCG14550.016.93296841
GGTCGCC14150.016.63727843
GATCTCG16200.016.29447234
AGAGCGT19500.016.0230888
GATCGGA21150.015.499141
ATCGGAA21550.015.415352
AAGAGCG24200.013.4567097
TCGGTGG20850.012.87141438
TCGGAAG26400.012.5834093
ATCTCGG21050.012.43566235
TCTCGGT20900.011.99865736
Produced by FastQC (version 0.11.2)