FastQCFastQC Report
Mon 6 Mar 2017
G150-M17-Index-18_GTCCGC-_BCALRAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M17-Index-18_GTCCGC-_BCALRAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21626067
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC10800.030.31716733
ACGTCCG11200.030.01915632
CTCGTAT12850.025.81907344
GATCGGA14050.023.840231
TCTCGTA13950.022.04862643
GTCCGCA15850.020.51901634
ATCGGAA16200.020.3898662
TCCGCAC17300.019.05246535
ACACGTC20600.016.7812113
CGTCTGA20350.016.77123816
ATCTCGT19900.016.33857542
GTCACGT22950.015.12778729
CGCACAT21300.014.85407837
ACGTCTG23700.014.58542115
CCGCACA22900.014.48913536
AGTCACG23550.014.27497728
CATCTCG23600.014.2427941
TCGGAAG25400.013.5242723
CGGAAGA29750.011.4733674
CACGTCT30900.011.25841714
Produced by FastQC (version 0.11.2)