FastQCFastQC Report
Mon 6 Mar 2017
G150-M1-Index-1_ATCACG-_BCALRAANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M1-Index-1_ATCACG-_BCALRAANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21943872
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT10850.022.9158711
CGTCGTG11600.021.24455612
AGCGTCG11800.020.69801310
GATCGGA14500.017.448621
TCGTGTA15000.016.8687314
ATCGGAA16000.015.5373812
GTCGTGT16450.014.84690213
CGTGTAG17950.014.34171715
GATCTCG16250.012.86029634
GAGCGTC20200.012.7444819
AGAGCGT23300.011.3321428
TGGTCGC16900.011.3257742
ATCTCGG18950.011.26012635
TCGGAAG24000.010.725043
GTGGTCG18600.010.4075541
GTCGCCG20550.09.42203444
AAGAGCG27700.09.2937887
CGGAAGA30450.08.5258144
GGTCGCC24900.08.30587743
TCTCGGT27050.08.29494836
Produced by FastQC (version 0.11.2)