G121-M5_ACAGTG-_BC7MKJANXX_L007_001.R2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	G121-M5_ACAGTG-_BC7MKJANXX_L007_001.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26983163
Sequences flagged as poor quality	0
Sequence length	50
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	498392	1.8470481018107479	Illumina Single End PCR Primer 1 (100% over 50bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTCGTG	74395	43.30399	12
GCGTCGT	74235	43.266926	11
AGCGTCG	73995	43.263275	10
GATCGGA	74650	43.2406	1
GTCGCCG	56285	43.22876	44
ATCGGAA	74690	43.10781	2
TCGTGTA	74985	42.79468	14
GTCGTGT	75490	42.773056	13
GAGCGTC	74825	42.73617	9
GATCTCG	62760	42.685913	34
CGTGTAG	75360	42.62261	15
TGGTCGC	56630	42.59619	42
GTGGTCG	58325	42.541786	41
TCGGAAG	75900	42.46383	3
AGAGCGT	75500	42.460876	8
GGTCGCC	57230	42.45329	43
ATCTCGG	64060	42.348003	35
AAGAGCG	75520	42.25754	7
CGGAAGA	75595	42.233517	4
TCTCGGT	63310	42.140476	36