FastQCFastQC Report
Tue 17 Jan 2017
G121-M12_CTTGTA-_BC7MKJANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M12_CTTGTA-_BC7MKJANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25397701
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2421570.9534603151678965Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT371200.042.81962211
CGTCGTG372950.042.6954712
GATCGGA373650.042.656271
AGCGTCG372750.042.52758810
ATCGGAA375450.042.3986052
TCGTGTA375350.042.35347414
GTCGTGT379300.042.08036813
CGTGTAG381200.041.6746515
GAGCGTC379450.041.6720779
TGGTCGC285150.041.506842
GTGGTCG295900.041.4993841
AGAGCGT382450.041.4466828
GATCTCG317250.041.3650734
TCGGAAG388050.041.1296463
ATCTCGG324950.041.01395835
GTCGCCG293450.040.91031644
AAGAGCG385500.040.8852167
CGGAAGA390250.040.5595054
TCTCGGT324200.040.43695436
TCGGTGG334200.040.29903838
Produced by FastQC (version 0.11.2)