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        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.11

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        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2025-08-04, 08:48 based on data in:


        General Statistics

        Showing 453/453 rows and 8/10 columns.
        Sample NameM Reads Mapped% AssignedM Assigned% rRNA% mRNAInsert Size% AlignedM Aligned
        G221_M01_G221_M01
        62.9%
        7.4
        G221_M01_primary_unique
        0.9%
        85.4%
        270 bp
        G221_M01_sorted
        93.6%
        6.9
        G221_M01_statistics_for_all_accepted_reads
        18.4
        G221_M01_statistics_for_primary_reads
        15.9
        G221_M01_statistics_for_primary_unique_reads
        14.7
        G221_M02_G221_M02
        68.3%
        10.4
        G221_M02_primary_unique
        4.3%
        81.5%
        272 bp
        G221_M02_sorted
        91.3%
        9.5
        G221_M02_statistics_for_all_accepted_reads
        25.7
        G221_M02_statistics_for_primary_reads
        22.4
        G221_M02_statistics_for_primary_unique_reads
        20.8
        G221_M03_G221_M03
        62.5%
        11.5
        G221_M03_primary_unique
        2.0%
        85.4%
        255 bp
        G221_M03_sorted
        93.6%
        10.8
        G221_M03_statistics_for_all_accepted_reads
        28.0
        G221_M03_statistics_for_primary_reads
        24.7
        G221_M03_statistics_for_primary_unique_reads
        23.0
        G221_M04AD_G221_M04AD
        65.9%
        9.9
        G221_M04AD_sorted
        93.3%
        9.2
        G221_M04AD_statistics_for_all_accepted_reads
        24.4
        G221_M04AD_statistics_for_primary_reads
        21.3
        G221_M04AD_statistics_for_primary_unique_reads
        19.8
        G221_M04A_G221_M04A
        65.8%
        30.0
        G221_M04A_primary_unique
        0.8%
        85.0%
        289 bp
        G221_M04A_sorted
        93.3%
        28.0
        G221_M04A_statistics_for_all_accepted_reads
        74.2
        G221_M04A_statistics_for_primary_reads
        64.7
        G221_M04A_statistics_for_primary_unique_reads
        60.1
        G221_M04_G221_M04
        75.6%
        6.0
        G221_M04_primary_unique
        0.3%
        85.7%
        300 bp
        G221_M04_sorted
        93.5%
        5.6
        G221_M04_statistics_for_all_accepted_reads
        14.6
        G221_M04_statistics_for_primary_reads
        12.9
        G221_M04_statistics_for_primary_unique_reads
        11.9
        G221_M05AD_G221_M05AD
        68.7%
        10.3
        G221_M05AD_sorted
        93.3%
        9.6
        G221_M05AD_statistics_for_all_accepted_reads
        25.0
        G221_M05AD_statistics_for_primary_reads
        22.1
        G221_M05AD_statistics_for_primary_unique_reads
        20.6
        G221_M05A_G221_M05A
        68.7%
        23.5
        G221_M05A_primary_unique
        1.4%
        85.9%
        289 bp
        G221_M05A_sorted
        93.3%
        22.0
        G221_M05A_statistics_for_all_accepted_reads
        57.1
        G221_M05A_statistics_for_primary_reads
        50.6
        G221_M05A_statistics_for_primary_unique_reads
        47.1
        G221_M05_G221_M05
        79.7%
        6.3
        G221_M05_primary_unique
        0.2%
        86.1%
        310 bp
        G221_M05_sorted
        93.4%
        5.9
        G221_M05_statistics_for_all_accepted_reads
        15.1
        G221_M05_statistics_for_primary_reads
        13.5
        G221_M05_statistics_for_primary_unique_reads
        12.6
        G221_M06AD_G221_M06AD
        67.7%
        10.1
        G221_M06AD_sorted
        93.7%
        9.5
        G221_M06AD_statistics_for_all_accepted_reads
        24.3
        G221_M06AD_statistics_for_primary_reads
        21.7
        G221_M06AD_statistics_for_primary_unique_reads
        20.3
        G221_M06A_G221_M06A
        67.6%
        33.0
        G221_M06A_primary_unique
        1.6%
        86.4%
        278 bp
        G221_M06A_sorted
        93.7%
        30.9
        G221_M06A_statistics_for_all_accepted_reads
        79.0
        G221_M06A_statistics_for_primary_reads
        70.6
        G221_M06A_statistics_for_primary_unique_reads
        66.0
        G221_M06_G221_M06
        80.6%
        6.6
        G221_M06_primary_unique
        0.5%
        85.6%
        346 bp
        G221_M06_sorted
        93.0%
        6.1
        G221_M06_statistics_for_all_accepted_reads
        15.7
        G221_M06_statistics_for_primary_reads
        14.2
        G221_M06_statistics_for_primary_unique_reads
        13.2
        G221_M07AD_G221_M07AD
        68.8%
        10.3
        G221_M07AD_sorted
        93.7%
        9.7
        G221_M07AD_statistics_for_all_accepted_reads
        24.5
        G221_M07AD_statistics_for_primary_reads
        22.1
        G221_M07AD_statistics_for_primary_unique_reads
        20.6
        G221_M07A_G221_M07A
        68.8%
        42.0
        G221_M07A_primary_unique
        1.3%
        86.6%
        291 bp
        G221_M07A_sorted
        93.7%
        39.3
        G221_M07A_statistics_for_all_accepted_reads
        99.8
        G221_M07A_statistics_for_primary_reads
        89.7
        G221_M07A_statistics_for_primary_unique_reads
        84.0
        G221_M07_G221_M07
        80.6%
        7.1
        G221_M07_primary_unique
        0.3%
        86.3%
        324 bp
        G221_M07_sorted
        93.2%
        6.7
        G221_M07_statistics_for_all_accepted_reads
        17.0
        G221_M07_statistics_for_primary_reads
        15.4
        G221_M07_statistics_for_primary_unique_reads
        14.3
        G221_M08_G221_M08
        67.8%
        7.2
        G221_M08_primary_unique
        2.7%
        84.6%
        275 bp
        G221_M08_sorted
        92.6%
        6.6
        G221_M08_statistics_for_all_accepted_reads
        17.6
        G221_M08_statistics_for_primary_reads
        15.4
        G221_M08_statistics_for_primary_unique_reads
        14.3
        G221_M09_G221_M09
        66.5%
        7.4
        G221_M09_primary_unique
        0.9%
        86.6%
        264 bp
        G221_M09_sorted
        93.9%
        6.9
        G221_M09_statistics_for_all_accepted_reads
        17.7
        G221_M09_statistics_for_primary_reads
        15.8
        G221_M09_statistics_for_primary_unique_reads
        14.7
        G221_M10_G221_M10
        65.5%
        7.4
        G221_M10_primary_unique
        0.8%
        86.4%
        309 bp
        G221_M10_sorted
        93.9%
        6.9
        G221_M10_statistics_for_all_accepted_reads
        18.1
        G221_M10_statistics_for_primary_reads
        15.8
        G221_M10_statistics_for_primary_unique_reads
        14.7
        G221_M11_G221_M11
        68.4%
        7.2
        G221_M11_primary_unique
        0.8%
        87.8%
        350 bp
        G221_M11_sorted
        94.2%
        6.7
        G221_M11_statistics_for_all_accepted_reads
        17.5
        G221_M11_statistics_for_primary_reads
        15.4
        G221_M11_statistics_for_primary_unique_reads
        14.3
        G221_M12_G221_M12
        64.6%
        8.2
        G221_M12_primary_unique
        1.2%
        86.4%
        287 bp
        G221_M12_sorted
        93.8%
        7.7
        G221_M12_statistics_for_all_accepted_reads
        20.3
        G221_M12_statistics_for_primary_reads
        17.6
        G221_M12_statistics_for_primary_unique_reads
        16.4
        G221_M13_G221_M13
        65.2%
        11.4
        G221_M13_primary_unique
        0.5%
        86.4%
        299 bp
        G221_M13_sorted
        93.6%
        10.6
        G221_M13_statistics_for_all_accepted_reads
        28.4
        G221_M13_statistics_for_primary_reads
        24.5
        G221_M13_statistics_for_primary_unique_reads
        22.7
        G221_M14_G221_M14
        67.9%
        6.0
        G221_M14_primary_unique
        0.9%
        87.3%
        329 bp
        G221_M14_sorted
        94.2%
        5.7
        G221_M14_statistics_for_all_accepted_reads
        14.7
        G221_M14_statistics_for_primary_reads
        12.9
        G221_M14_statistics_for_primary_unique_reads
        12.1
        G221_M15_G221_M15
        69.7%
        7.9
        G221_M15_primary_unique
        0.8%
        87.1%
        372 bp
        G221_M15_sorted
        94.1%
        7.4
        G221_M15_statistics_for_all_accepted_reads
        19.0
        G221_M15_statistics_for_primary_reads
        16.9
        G221_M15_statistics_for_primary_unique_reads
        15.8
        G221_M16_G221_M16
        64.3%
        6.3
        G221_M16_primary_unique
        1.0%
        86.8%
        284 bp
        G221_M16_sorted
        93.7%
        5.9
        G221_M16_statistics_for_all_accepted_reads
        15.6
        G221_M16_statistics_for_primary_reads
        13.7
        G221_M16_statistics_for_primary_unique_reads
        12.7
        G221_M17_G221_M17
        63.8%
        6.5
        G221_M17_primary_unique
        1.0%
        87.8%
        273 bp
        G221_M17_sorted
        94.1%
        6.2
        G221_M17_statistics_for_all_accepted_reads
        16.2
        G221_M17_statistics_for_primary_reads
        14.1
        G221_M17_statistics_for_primary_unique_reads
        13.1
        G221_M18_G221_M18
        66.1%
        7.1
        G221_M18_primary_unique
        0.7%
        88.5%
        279 bp
        G221_M18_sorted
        94.4%
        6.7
        G221_M18_statistics_for_all_accepted_reads
        17.5
        G221_M18_statistics_for_primary_reads
        15.3
        G221_M18_statistics_for_primary_unique_reads
        14.2
        G221_M19_G221_M19
        65.2%
        7.0
        G221_M19_primary_unique
        0.8%
        88.1%
        289 bp
        G221_M19_sorted
        94.8%
        6.6
        G221_M19_statistics_for_all_accepted_reads
        17.2
        G221_M19_statistics_for_primary_reads
        15.0
        G221_M19_statistics_for_primary_unique_reads
        13.9
        G221_M20_G221_M20
        65.3%
        6.2
        G221_M20_primary_unique
        0.6%
        89.0%
        293 bp
        G221_M20_sorted
        94.9%
        5.8
        G221_M20_statistics_for_all_accepted_reads
        15.0
        G221_M20_statistics_for_primary_reads
        13.2
        G221_M20_statistics_for_primary_unique_reads
        12.3
        G221_M21_G221_M21
        62.7%
        6.0
        G221_M21_primary_unique
        0.5%
        87.2%
        270 bp
        G221_M21_sorted
        94.3%
        5.6
        G221_M21_statistics_for_all_accepted_reads
        14.8
        G221_M21_statistics_for_primary_reads
        12.9
        G221_M21_statistics_for_primary_unique_reads
        11.9
        G221_M22_G221_M22
        63.5%
        6.1
        G221_M22_primary_unique
        0.6%
        87.1%
        263 bp
        G221_M22_sorted
        94.0%
        5.7
        G221_M22_statistics_for_all_accepted_reads
        15.0
        G221_M22_statistics_for_primary_reads
        13.2
        G221_M22_statistics_for_primary_unique_reads
        12.2
        G221_M23_G221_M23
        64.8%
        6.1
        G221_M23_primary_unique
        0.7%
        86.2%
        286 bp
        G221_M23_sorted
        93.4%
        5.7
        G221_M23_statistics_for_all_accepted_reads
        15.1
        G221_M23_statistics_for_primary_reads
        13.1
        G221_M23_statistics_for_primary_unique_reads
        12.2
        G221_M24_G221_M24
        60.8%
        6.9
        G221_M24_primary_unique
        0.5%
        84.8%
        273 bp
        G221_M24_sorted
        92.6%
        6.4
        G221_M24_statistics_for_all_accepted_reads
        18.2
        G221_M24_statistics_for_primary_reads
        15.3
        G221_M24_statistics_for_primary_unique_reads
        13.9
        G221_M25_G221_M25
        67.2%
        10.8
        G221_M25_primary_unique
        0.7%
        85.1%
        305 bp
        G221_M25_sorted
        93.4%
        10.1
        G221_M25_statistics_for_all_accepted_reads
        26.7
        G221_M25_statistics_for_primary_reads
        23.3
        G221_M25_statistics_for_primary_unique_reads
        21.6
        G221_M26_G221_M26
        66.3%
        8.1
        G221_M26_primary_unique
        0.7%
        84.7%
        297 bp
        G221_M26_sorted
        93.1%
        7.6
        G221_M26_statistics_for_all_accepted_reads
        20.3
        G221_M26_statistics_for_primary_reads
        17.6
        G221_M26_statistics_for_primary_unique_reads
        16.3
        G221_M27_G221_M27
        65.0%
        8.8
        G221_M27_primary_unique
        0.7%
        85.0%
        283 bp
        G221_M27_sorted
        93.3%
        8.2
        G221_M27_statistics_for_all_accepted_reads
        22.1
        G221_M27_statistics_for_primary_reads
        19.1
        G221_M27_statistics_for_primary_unique_reads
        17.7
        G221_M28_G221_M28
        59.3%
        6.0
        G221_M28_primary_unique
        1.4%
        86.4%
        264 bp
        G221_M28_sorted
        93.7%
        5.6
        G221_M28_statistics_for_all_accepted_reads
        14.9
        G221_M28_statistics_for_primary_reads
        13.0
        G221_M28_statistics_for_primary_unique_reads
        12.0
        G221_M29_G221_M29
        64.3%
        7.9
        G221_M29_primary_unique
        0.7%
        80.9%
        189 bp
        G221_M29_sorted
        88.8%
        7.0
        G221_M29_statistics_for_all_accepted_reads
        20.2
        G221_M29_statistics_for_primary_reads
        17.5
        G221_M29_statistics_for_primary_unique_reads
        15.8
        G221_M30_G221_M30
        65.9%
        8.5
        G221_M30_primary_unique
        0.9%
        78.4%
        204 bp
        G221_M30_sorted
        87.6%
        7.4
        G221_M30_statistics_for_all_accepted_reads
        21.8
        G221_M30_statistics_for_primary_reads
        18.9
        G221_M30_statistics_for_primary_unique_reads
        16.9
        G221_M31_G221_M31
        67.1%
        7.5
        G221_M31_primary_unique
        0.7%
        79.5%
        215 bp
        G221_M31_sorted
        87.6%
        6.6
        G221_M31_statistics_for_all_accepted_reads
        19.4
        G221_M31_statistics_for_primary_reads
        16.8
        G221_M31_statistics_for_primary_unique_reads
        15.1
        G221_M32_G221_M32
        62.0%
        9.2
        G221_M32_primary_unique
        1.0%
        78.8%
        177 bp
        G221_M32_sorted
        89.8%
        8.2
        G221_M32_statistics_for_all_accepted_reads
        22.9
        G221_M32_statistics_for_primary_reads
        20.0
        G221_M32_statistics_for_primary_unique_reads
        18.3
        G221_M33_G221_M33
        66.1%
        5.9
        G221_M33_primary_unique
        0.5%
        82.6%
        218 bp
        G221_M33_sorted
        91.3%
        5.4
        G221_M33_statistics_for_all_accepted_reads
        14.9
        G221_M33_statistics_for_primary_reads
        13.0
        G221_M33_statistics_for_primary_unique_reads
        11.9
        G221_M34_G221_M34
        63.6%
        5.0
        G221_M34_primary_unique
        0.7%
        81.9%
        223 bp
        G221_M34_sorted
        89.9%
        4.5
        G221_M34_statistics_for_all_accepted_reads
        12.9
        G221_M34_statistics_for_primary_reads
        11.0
        G221_M34_statistics_for_primary_unique_reads
        9.9
        G221_M35_G221_M35
        61.3%
        7.3
        G221_M35_primary_unique
        1.1%
        70.8%
        185 bp
        G221_M35_sorted
        81.8%
        6.0
        G221_M35_statistics_for_all_accepted_reads
        19.8
        G221_M35_statistics_for_primary_reads
        16.9
        G221_M35_statistics_for_primary_unique_reads
        14.7
        G221_M36_G221_M36
        63.1%
        5.2
        G221_M36_primary_unique
        0.7%
        78.9%
        201 bp
        G221_M36_sorted
        85.9%
        4.5
        G221_M36_statistics_for_all_accepted_reads
        14.0
        G221_M36_statistics_for_primary_reads
        11.9
        G221_M36_statistics_for_primary_unique_reads
        10.4
        G221_M37_G221_M37
        60.2%
        5.8
        G221_M37_primary_unique
        0.8%
        62.1%
        181 bp
        G221_M37_sorted
        74.5%
        4.3
        G221_M37_statistics_for_all_accepted_reads
        16.5
        G221_M37_statistics_for_primary_reads
        13.9
        G221_M37_statistics_for_primary_unique_reads
        11.5
        G221_M38_G221_M38
        61.4%
        9.0
        G221_M38_primary_unique
        1.5%
        64.4%
        189 bp
        G221_M38_sorted
        76.6%
        6.9
        G221_M38_statistics_for_all_accepted_reads
        26.1
        G221_M38_statistics_for_primary_reads
        21.5
        G221_M38_statistics_for_primary_unique_reads
        18.0
        G221_M39_G221_M39
        65.1%
        10.3
        G221_M39_primary_unique
        5.5%
        72.6%
        197 bp
        G221_M39_sorted
        83.0%
        8.6
        G221_M39_statistics_for_all_accepted_reads
        26.7
        G221_M39_statistics_for_primary_reads
        23.3
        G221_M39_statistics_for_primary_unique_reads
        20.7
        G221_M40_G221_M40
        62.9%
        6.1
        G221_M40_primary_unique
        1.5%
        83.0%
        188 bp
        G221_M40_sorted
        89.5%
        5.5
        G221_M40_statistics_for_all_accepted_reads
        15.2
        G221_M40_statistics_for_primary_reads
        13.3
        G221_M40_statistics_for_primary_unique_reads
        12.2
        G221_M41_G221_M41
        64.6%
        9.5
        G221_M41_primary_unique
        0.7%
        81.3%
        192 bp
        G221_M41_sorted
        89.0%
        8.4
        G221_M41_statistics_for_all_accepted_reads
        24.2
        G221_M41_statistics_for_primary_reads
        21.1
        G221_M41_statistics_for_primary_unique_reads
        19.0
        G221_M42_G221_M42
        67.7%
        5.2
        G221_M42_primary_unique
        0.8%
        83.1%
        229 bp
        G221_M42_sorted
        90.0%
        4.7
        G221_M42_statistics_for_all_accepted_reads
        13.2
        G221_M42_statistics_for_primary_reads
        11.5
        G221_M42_statistics_for_primary_unique_reads
        10.4
        G221_M43_G221_M43
        61.9%
        7.3
        G221_M43_primary_unique
        3.3%
        72.3%
        193 bp
        G221_M43_sorted
        80.6%
        5.9
        G221_M43_statistics_for_all_accepted_reads
        20.3
        G221_M43_statistics_for_primary_reads
        17.0
        G221_M43_statistics_for_primary_unique_reads
        14.6
        G221_M44_G221_M44
        67.6%
        5.4
        G221_M44_primary_unique
        4.6%
        77.6%
        194 bp
        G221_M44_sorted
        87.4%
        4.7
        G221_M44_statistics_for_all_accepted_reads
        13.3
        G221_M44_statistics_for_primary_reads
        11.8
        G221_M44_statistics_for_primary_unique_reads
        10.8
        G221_M45_G221_M45
        69.0%
        5.4
        G221_M45_primary_unique
        0.5%
        85.6%
        219 bp
        G221_M45_sorted
        90.8%
        4.9
        G221_M45_statistics_for_all_accepted_reads
        13.3
        G221_M45_statistics_for_primary_reads
        11.9
        G221_M45_statistics_for_primary_unique_reads
        10.8
        G221_M46_G221_M46
        71.7%
        6.5
        G221_M46_primary_unique
        0.6%
        87.3%
        233 bp
        G221_M46_sorted
        93.6%
        6.1
        G221_M46_statistics_for_all_accepted_reads
        15.1
        G221_M46_statistics_for_primary_reads
        13.8
        G221_M46_statistics_for_primary_unique_reads
        13.0
        G221_M47_G221_M47
        71.5%
        6.2
        G221_M47_primary_unique
        0.7%
        86.5%
        242 bp
        G221_M47_sorted
        92.4%
        5.7
        G221_M47_statistics_for_all_accepted_reads
        14.7
        G221_M47_statistics_for_primary_reads
        13.3
        G221_M47_statistics_for_primary_unique_reads
        12.4
        G221_M48_G221_M48
        65.3%
        7.6
        G221_M48_primary_unique
        2.5%
        85.9%
        199 bp
        G221_M48_sorted
        92.5%
        7.1
        G221_M48_statistics_for_all_accepted_reads
        18.1
        G221_M48_statistics_for_primary_reads
        16.4
        G221_M48_statistics_for_primary_unique_reads
        15.3
        G221_M49_G221_M49
        67.4%
        10.1
        G221_M49_primary_unique
        1.0%
        85.0%
        273 bp
        G221_M49_sorted
        93.0%
        9.4
        G221_M49_statistics_for_all_accepted_reads
        24.6
        G221_M49_statistics_for_primary_reads
        21.6
        G221_M49_statistics_for_primary_unique_reads
        20.1
        G221_M50_G221_M50
        65.7%
        10.8
        G221_M50_primary_unique
        1.0%
        86.6%
        275 bp
        G221_M50_sorted
        93.8%
        10.1
        G221_M50_statistics_for_all_accepted_reads
        26.4
        G221_M50_statistics_for_primary_reads
        23.1
        G221_M50_statistics_for_primary_unique_reads
        21.6
        G221_M51_G221_M51
        62.2%
        2.9
        G221_M51_primary_unique
        1.6%
        85.7%
        282 bp
        G221_M51_sorted
        93.2%
        2.7
        G221_M51_statistics_for_all_accepted_reads
        7.3
        G221_M51_statistics_for_primary_reads
        6.3
        G221_M51_statistics_for_primary_unique_reads
        5.8
        G221_M52_G221_M52
        61.0%
        6.1
        G221_M52_primary_unique
        1.6%
        85.8%
        248 bp
        G221_M52_sorted
        93.5%
        5.7
        G221_M52_statistics_for_all_accepted_reads
        15.2
        G221_M52_statistics_for_primary_reads
        13.2
        G221_M52_statistics_for_primary_unique_reads
        12.3
        G221_M53_G221_M53
        63.0%
        6.5
        G221_M53_primary_unique
        1.1%
        86.9%
        254 bp
        G221_M53_sorted
        94.2%
        6.1
        G221_M53_statistics_for_all_accepted_reads
        15.9
        G221_M53_statistics_for_primary_reads
        13.9
        G221_M53_statistics_for_primary_unique_reads
        13.0
        G221_M54_G221_M54
        73.4%
        6.0
        G221_M54_primary_unique
        0.8%
        83.7%
        241 bp
        G221_M54_sorted
        92.7%
        5.6
        G221_M54_statistics_for_all_accepted_reads
        14.2
        G221_M54_statistics_for_primary_reads
        12.9
        G221_M54_statistics_for_primary_unique_reads
        12.0
        G221_M55_G221_M55
        70.6%
        5.9
        G221_M55_primary_unique
        0.7%
        87.2%
        265 bp
        G221_M55_sorted
        94.4%
        5.6
        G221_M55_statistics_for_all_accepted_reads
        14.0
        G221_M55_statistics_for_primary_reads
        12.5
        G221_M55_statistics_for_primary_unique_reads
        11.8
        G221_M56_G221_M56
        70.8%
        6.7
        G221_M56_primary_unique
        1.0%
        86.4%
        260 bp
        G221_M56_sorted
        93.6%
        6.3
        G221_M56_statistics_for_all_accepted_reads
        15.8
        G221_M56_statistics_for_primary_reads
        14.3
        G221_M56_statistics_for_primary_unique_reads
        13.5
        G221_M57_G221_M57
        63.0%
        5.7
        G221_M57_primary_unique
        0.6%
        86.5%
        249 bp
        G221_M57_sorted
        93.8%
        5.3
        G221_M57_statistics_for_all_accepted_reads
        14.2
        G221_M57_statistics_for_primary_reads
        12.3
        G221_M57_statistics_for_primary_unique_reads
        11.4
        G221_M58_G221_M58
        69.2%
        6.0
        G221_M58_primary_unique
        0.6%
        85.8%
        280 bp
        G221_M58_sorted
        93.5%
        5.6
        G221_M58_statistics_for_all_accepted_reads
        14.5
        G221_M58_statistics_for_primary_reads
        12.8
        G221_M58_statistics_for_primary_unique_reads
        11.9
        G221_M59_G221_M59
        63.4%
        7.1
        G221_M59_primary_unique
        1.3%
        86.7%
        265 bp
        G221_M59_sorted
        93.7%
        6.6
        G221_M59_statistics_for_all_accepted_reads
        17.4
        G221_M59_statistics_for_primary_reads
        15.3
        G221_M59_statistics_for_primary_unique_reads
        14.1
        G221_M60_G221_M60
        61.0%
        6.9
        G221_M60_primary_unique
        0.8%
        86.5%
        231 bp
        G221_M60_sorted
        94.1%
        6.5
        G221_M60_statistics_for_all_accepted_reads
        16.9
        G221_M60_statistics_for_primary_reads
        14.8
        G221_M60_statistics_for_primary_unique_reads
        13.8
        G221_M61_G221_M61
        68.5%
        7.2
        G221_M61_primary_unique
        0.8%
        84.9%
        244 bp
        G221_M61_sorted
        92.9%
        6.7
        G221_M61_statistics_for_all_accepted_reads
        17.5
        G221_M61_statistics_for_primary_reads
        15.5
        G221_M61_statistics_for_primary_unique_reads
        14.4
        G221_M62_G221_M62
        67.5%
        17.3
        G221_M62_primary_unique
        1.4%
        85.2%
        249 bp
        G221_M62_sorted
        93.2%
        16.1
        G221_M62_statistics_for_all_accepted_reads
        41.8
        G221_M62_statistics_for_primary_reads
        37.2
        G221_M62_statistics_for_primary_unique_reads
        34.5
        G221_M63_G221_M63
        71.5%
        8.5
        G221_M63_primary_unique
        0.9%
        84.7%
        221 bp
        G221_M63_sorted
        92.3%
        7.8
        G221_M63_statistics_for_all_accepted_reads
        20.2
        G221_M63_statistics_for_primary_reads
        18.3
        G221_M63_statistics_for_primary_unique_reads
        17.0
        G221_M64_G221_M64
        68.3%
        5.4
        G221_M64_primary_unique
        0.7%
        84.5%
        202 bp
        G221_M64_sorted
        91.9%
        4.9
        G221_M64_statistics_for_all_accepted_reads
        12.8
        G221_M64_statistics_for_primary_reads
        11.5
        G221_M64_statistics_for_primary_unique_reads
        10.7
        G221_M65_G221_M65
        69.6%
        6.7
        G221_M65_primary_unique
        0.9%
        86.6%
        217 bp
        G221_M65_sorted
        93.7%
        6.3
        G221_M65_statistics_for_all_accepted_reads
        15.7
        G221_M65_statistics_for_primary_reads
        14.4
        G221_M65_statistics_for_primary_unique_reads
        13.5
        G221_M66_G221_M66
        74.5%
        7.2
        G221_M66_primary_unique
        0.8%
        85.0%
        238 bp
        G221_M66_sorted
        92.9%
        6.7
        G221_M66_statistics_for_all_accepted_reads
        16.6
        G221_M66_statistics_for_primary_reads
        15.3
        G221_M66_statistics_for_primary_unique_reads
        14.3
        G221_M67_G221_M67
        73.3%
        6.7
        G221_M67_primary_unique
        0.6%
        85.7%
        234 bp
        G221_M67_sorted
        93.4%
        6.3
        G221_M67_statistics_for_all_accepted_reads
        15.7
        G221_M67_statistics_for_primary_reads
        14.4
        G221_M67_statistics_for_primary_unique_reads
        13.5
        G221_M68_G221_M68
        73.3%
        6.0
        G221_M68_primary_unique
        0.5%
        84.2%
        233 bp
        G221_M68_sorted
        91.7%
        5.5
        G221_M68_statistics_for_all_accepted_reads
        14.3
        G221_M68_statistics_for_primary_reads
        12.9
        G221_M68_statistics_for_primary_unique_reads
        11.9
        statistics_for_primary_unique_reads
        11.9

        RSeQC

        RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput RNA-seq data.

        Infer experiment

        Infer experiment counts the percentage of reads and read pairs that match the strandedness of overlapping transcripts. It can be used to infer whether RNA-seq library preps are stranded (sense or antisense).

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        featureCounts

        Subread featureCounts is a highly efficient general-purpose read summarization program that counts mapped reads for genomic features such as genes, exons, promoter, gene bodies, genomic bins and chromosomal locations.

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        Picard

        Picard is a set of Java command line tools for manipulating high-throughput sequencing data.

        Insert Size

        Plot shows the number of reads at a given insert size. Reads with different orientations are summed.

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        RnaSeqMetrics Assignment

        Number of bases in primary alignments that align to regions in the reference genome.

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        RnaSeqMetrics Strand Mapping

        Number of aligned reads that map to the correct strand.

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        Gene Coverage

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        Samtools

        Samtools is a suite of programs for interacting with high-throughput sequencing data.

        Samtools Flagstat

        This module parses the output from samtools flagstat. All numbers in millions.

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        STAR

        STAR is an ultrafast universal RNA-seq aligner.

        Alignment Scores

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