FastQCFastQC Report
Wed 13 Sep 2023
SRR12649919_pass.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12649919_pass.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34163094
Sequences flagged as poor quality0
Sequence length35-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACT5544001.622803836209917No Hit
CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTC5148611.5070678317367858No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG4471301.308810027569517No Hit
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG3853851.128074055587588No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT3821851.1187072224781514No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC3326060.9735827791241625No Hit
CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC3255750.953002090501522No Hit
CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTC3060960.8959844210831723No Hit
CTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTAT3056720.894743315696172No Hit
CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG2738950.8017277357841184No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT2558820.7490012467840296No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG2252310.659281621272359No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC2216460.6487878410544432No Hit
CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG1910970.5593667833481358No Hit
TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGG1843420.5395939840811842No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC1692370.49537960466929604No Hit
CCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGT1565250.45816986014205857No Hit
CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT1527180.44702625587717554No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGC1465430.4289511951113093No Hit
CTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTG1450290.424519512196407No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT1450030.42444340667739283No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCC1422530.41639378447397063No Hit
CTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTT1378020.4033651050458135No Hit
CACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATT1364930.3995334848769845No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG1323910.3875263756848253No Hit
CCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA1297720.37986020821182065No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT1263100.36972646564154876No Hit
CTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTG1195780.3500209904875712No Hit
CTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCG1195480.3499331764271702No Hit
GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG1175620.344119885628626No Hit
GTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTCC1170530.34262997373715626No Hit
CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT1169890.3424426370749675No Hit
CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT1168730.3421030893747504No Hit
ATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGCATTTG1071550.3136571880755297No Hit
CGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC1012640.29641343374812595No Hit
CGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT1007990.29505231581191094No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCG998300.2922159216609596No Hit
CACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCC994510.2911065373645607No Hit
GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG955400.279658511023621No Hit
CGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTG879570.25746204368960257No Hit
CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC854250.2500505369917608No Hit
CTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCAT850850.2490553109738831No Hit
CGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCG839630.24577106511488683No Hit
CTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGC812960.23796439514524062No Hit
CTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTC797350.23339513686904353No Hit
CACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCC789220.23101537583217727No Hit
GTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCT774530.22671541400787645No Hit
CCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGG762860.22329944705827875No Hit
GATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT749740.21945904548340966No Hit
ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGG727380.21291397084819072No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACC703050.2057922505496721No Hit
ATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC698190.20436966277117638No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG697240.20409158491324No Hit
GCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG682730.19984431152517976No Hit
CTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGC670920.19638736468072826No Hit
GGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTT670720.19632882197379428No Hit
CTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCT669910.19609172401071168No Hit
GGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC666660.1951404050230345No Hit
CGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCCGGG658200.19266404851972715No Hit
GTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGC657590.19248549326357853No Hit
CCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATAC655360.19183274208126466No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG653990.1914317245387669No Hit
CAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCAA653270.19122097079380457No Hit
CGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGT646610.18927149865290302No Hit
CACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGT627770.18375677565972215No Hit
CTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC609280.17834450240367575No Hit
CTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTAC596280.17453922645296704No Hit
CTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGG589300.17249608598097116No Hit
CGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTT586150.17157403834676097No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC583850.17090079721702023No Hit
GGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTC581900.1703300058244139No Hit
CGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGC579010.16948406370921792No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCAT574980.16830442816449823No Hit
CTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACG566480.16581636311980408No Hit
CTTTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGGC561940.16448744367240273No Hit
CTTTGGTTTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTAT558220.16339854932343073No Hit
CACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCC540320.15815897705283954No Hit
CTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTC535540.15675980635711742No Hit
CACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAAT534120.1563441531378862No Hit
CTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCC526080.15399073631914018No Hit
CACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGC524210.15344336200930747No Hit
CTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACAC502460.14707684263023718No Hit
CCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGC502090.14696853862240933No Hit
CCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTT496530.14534105136964467No Hit
GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGC493790.14453901628464916No Hit
CGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTT493580.1444775464423685No Hit
CCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGT492100.14404433041105702No Hit
GTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC485180.14201875275114134No Hit
CAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCGC479580.1403795569569899No Hit
ACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCCGG476630.1395160520297137No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA474670.13894233350176072No Hit
CATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCA463860.13577810019197908No Hit
CTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATT461990.13523072588214638No Hit
CTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGGCCTTC450360.13182646747393548No Hit
CCGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGT445670.13045364099633366No Hit
GTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCC438900.12847197036661842No Hit
CGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCACGT432360.12655762384987732No Hit
CCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGG429030.12558288777942653No Hit
GCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTT427580.1251584531541552No Hit
CTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACAC426640.12488330243156547No Hit
CGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTG420090.12296602877947763No Hit
GTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCT418790.12258550118440677No Hit
TCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGT413710.12109851642828368No Hit
ATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTT411500.12045161951666321No Hit
CGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGAT407900.11939785079185158No Hit
CCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTC407030.11914319001668876No Hit
CAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTAC403580.11813332832207761No Hit
TGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTCCC402700.11787574041156812No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG402050.11768547661403267No Hit
CCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTC400550.11724640631202783No Hit
CTCGGTACTACATGCTTAGTCAGTCTTTACATTCGCTTGCCAGCTGCGG399580.11696247418339803No Hit
CTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTTAT395750.11584138134561231No Hit
CCCCGCTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT395330.11571844166105097No Hit
GCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTT394090.11535547687806028No Hit
CCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC392530.11489884376397524No Hit
GCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCT390540.11431634382998214No Hit
CCCGCTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG385910.11296108016446052No Hit
CGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCT385460.11282935907385908No Hit
CCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAGGT384720.11261275105820334No Hit
AACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGG384040.11241370585462779No Hit
CTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAGT364830.10679067885361905No Hit
GTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAGGGCACAACCTC364020.10655358089053645No Hit
CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC361550.10583057845990179No Hit
CGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATC357390.10461289015567501No Hit
TCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTC355820.10415332990624326No Hit
CTTTCACCCCCAGCCACAAGTCATCCGCTAATTTTTCAACATTAGTCGGT354400.10373767668701202No Hit
CCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTG353880.10358546564898366No Hit
CGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCATTCGC351000.10274245066913434No Hit
AGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGC349990.10244680999911776No Hit
GTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCG347740.10178820454611048No Hit
GCTGCTTCTAAGCCAACATCCTGGCTGTCTGGGCCTTCCCACATCGTTTC346280.10136084278549243No Hit
CGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTC345640.1011735061233037No Hit
CCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGC345560.10115008904053012No Hit
AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC345530.10114130763449No Hit
CTGGTATCTTCGACTGATTTCAGCTCCATCCGCGAGGGACCTCACCTAC342350.10021047859423973No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)