FastQCFastQC Report
Wed 13 Sep 2023
SRR12649887_pass.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12649887_pass.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27462982
Sequences flagged as poor quality0
Sequence length35-50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCAGTC1573530.5729639993209769No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1105310.4024726812259499No Hit
CCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCAGT1054170.3838512511132258No Hit
CTTCCGTACGCCACATTTCCCACGCCGCGACGCGCGGCGGGGATTCGGCG999100.3637988037861293No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG978000.3561157342636717No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT961920.35026057985982734No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG934440.3402543831547499No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT850240.3095949303684502No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG835970.3043988449615559No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCC814500.29658104862756707No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG788730.28719750826767465No Hit
CACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGC756060.27530149493598327No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG692530.2521685372695507No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCC687910.2504862727579984No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG684590.24927737271939368No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC670190.24403395086520463No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT656130.2389143320270173No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT628750.22894454797370511No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGG625650.22781575576898386No Hit
CGCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACG594340.21641495450129924No Hit
CGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGA587400.21388791646879424No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG574360.20913970667861195No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCG547700.1994320937180092No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACC538670.1961440312636115No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT529990.19298341309039202No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT523040.1904527337927105No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACC519520.18917100845057538No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCC514960.1875105915300822No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC511940.18641092944677312No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG494510.1800642042440985No Hit
CACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTG487750.17760270898477085No Hit
CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTC482360.17564006705462648No Hit
CCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGT472190.17193690037010548No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG457970.1667590212890938No Hit
CCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGG453200.1650221377998937No Hit
CTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGC453050.1649675188222459No Hit
CCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTAC451610.16444317663682698No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTAC450720.16411910403611668No Hit
GCTCCTTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTT445450.16220015728809056No Hit
CCGACTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTC436090.15879193308286768No Hit
CCGCCGTTTACCCGCGCTTCATTGAATTTCTTCACTTTGACATTCAGAGC431980.1572953730953179No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC428820.15614473329953754No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG424910.15472099861551816No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT424160.15444790372727915No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC418430.15236145878113308No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC412630.1502495249787514No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCT410920.14962686863356645No Hit
CACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGG408540.14876024752155464No Hit
CTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTC395780.14411399315631493No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT395120.1438736696546646No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT393990.14346220668971782No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC391370.14250819521346955No Hit
CTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAAC388890.14160516144969254No Hit
CCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGC384580.14003577615861235No Hit
CCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC381300.13884144118071373No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG380970.13872127942988857No Hit
CGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGT370670.1349707762980728No Hit
CCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCC367630.13386383168441068No Hit
CCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGAC355290.1293705104565848No Hit
CCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCT354300.1290100252041093No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGA354260.1289954601434032No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT352150.12822715319115746No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACC350270.12754259533797166No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT348930.12705466580431796No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG348530.12690901519725717No Hit
CCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGG348220.12679613597678505No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA348150.12677064712054942No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTACGCTCGTGCTC346490.12616619710124705No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCG344240.12534691243653No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC343000.12489539555464152No Hit
CGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCC341230.12425089161839745No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC340680.12405062203368884No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAG334030.12162918069130292No Hit
CACCTTTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGC330660.12040207432681563No Hit
GTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCC330420.12031468396257916No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCA329980.12015446829481227No Hit
GTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTT328960.11978305924680722No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACC326280.1188072001794998No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC320450.11668434258158855No Hit
CTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGT318650.11602891484981492No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT314780.11461974522650163No Hit
CCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAG312590.11382230815284371No Hit
CGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC312480.11378225423590198No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT310660.11311954397377531No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTT309420.11266802709188682No Hit
CTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTT308960.11250052889376688No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC304120.11073815654833113No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG303150.11038495382620866No Hit
CTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAA298040.10852426732100688No Hit
CCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGG296390.10792345856688104No Hit
CCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCAC295980.10777416669464372No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG294510.10723890071369524No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT294030.10706411998522229No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC290170.1056585916270855No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG288910.10519979221484396No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCA286260.10423485694306613No Hit
GTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCG284900.10373964487905937No Hit
CTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGA281360.10245063700657125No Hit
CCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTT280500.10213748820139051No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC278750.10150026679549948No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC278250.10131820353667348No Hit
CCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTT276710.10075744869948937No Hit
GCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTC275760.10041152850771995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)