FastQCFastQC Report
Wed 13 Sep 2023
SRR12649882_pass.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12649882_pass.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93315431
Sequences flagged as poor quality0
Sequence length35-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTC18816632.0164542775352983No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACT17880821.9161696847330643No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG14807971.5868725934513446No Hit
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG13680961.4660983562300645No Hit
CTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTAT12209591.3084213263720552No Hit
CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTC10795471.156879401864414No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT10652541.1415625353538794No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC9860091.0566408893294401No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT9854981.056093284292927No Hit
CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC9430731.0106292066528633No Hit
CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG7547800.8088480028560335No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG6354110.6809281093070234No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC6265130.6713927088864863No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGC5576570.5976042697589855No Hit
CTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTT5181550.5552725786585072No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC5033110.539365241746566No Hit
CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG4853680.5201369106895085No Hit
CTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTG4539580.48647688290696534No Hit
CTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTG4338960.4649777591446799No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT4134570.4430746293182743No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCC4114870.44096351009727425No Hit
CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT4101850.4395682424699941No Hit
GTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTCC3996010.42822606691920007No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT3978550.4263549937416031No Hit
TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGG3910010.41901001346711886No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG3834210.41088702681981937No Hit
CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT3832610.4107155653602457No Hit
CCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGT3735770.4003378605195533No Hit
CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT3701370.3966514391387208No Hit
CTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCG3424280.36695752924293945No Hit
GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG3390610.36334933715303747No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCG3385300.3627802994340775No Hit
CCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA3348310.35881632481556025No Hit
CACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATT3108210.3330863895382962No Hit
CACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCC3063270.32827046579252256No Hit
ATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGCATTTG3005350.32206356095595806No Hit
CGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT2851480.305574326715589No Hit
ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGG2820980.3023058426424671No Hit
CTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCAT2719180.29139660727709654No Hit
CTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTC2694230.28872288014187064No Hit
CGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC2580130.27649553480602795No Hit
CTTTGGTTTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTAT2568550.27525458249236395No Hit
GTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCT2510800.2690658954358792No Hit
CGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGT2473700.2650901328420162No Hit
CGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTG2452580.2628268415756447No Hit
CTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGG2448820.26242390714564673No Hit
CTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGC2420780.2594190450666192No Hit
CTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCT2299690.2464426274792644No Hit
CGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCG2269940.2432545159653177No Hit
GATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT2216240.23749984072837857No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG2194840.23520654370658162No Hit
CTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2186190.23427958019076178No Hit
CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC2120560.22724644544587702No Hit
GCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG2118400.22701497247545263No Hit
GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG2088780.22384079220509628No Hit
CCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATAC2071350.2219729339298663No Hit
ATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2052750.21997969446232316No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACC2016830.21613038469489573No Hit
CACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCC1984120.2126250694807379No Hit
CTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACG1973850.2115245012370998No Hit
CAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCAA1914740.20519007193997743No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG1908060.20447422034625762No Hit
GGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTC1883390.2018304989664571No Hit
CCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGG1867450.20012231417545506No Hit
CTTTCACCCCCAGCCACAAGTCATCCGCTAATTTTTCAACATTAGTCGGT1857620.19906889783319975No Hit
GGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC1837890.19695456370983272No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCAT1835970.19674880995834443No Hit
GTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGC1808570.19381253246314642No Hit
CGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCCGGG1718390.18414853594792913No Hit
CGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTT1717820.18408745280295602No Hit
CTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTAC1700190.18219816184527937No Hit
CACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCC1678020.17982234899606261No Hit
CTTTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGGC1674840.17948156934516007No Hit
CAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCGC1669590.17891896143093416No Hit
CTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGC1611040.17264454364466258No Hit
GTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC1604470.17194048002628848No Hit
GGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTT1575120.16879523387723516No Hit
GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGC1573290.16859912483284784No Hit
CTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATT1557690.1669273756020052No Hit
CTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCA1520960.16299126347066864No Hit
CTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACAC1519210.16280372749926No Hit
CCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGT1517920.1626654866974788No Hit
CACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGT1516470.1625100997497402No Hit
ATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTC1488950.15956096264507422No Hit
CTCTTTGTATGCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCC1488820.15954703140148385No Hit
GCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTT1487600.15941629203855898No Hit
ATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTT1458840.15633427230272343No Hit
CCGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGT1452010.15560234619716862No Hit
CATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCA1441100.1544331933697011No Hit
AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC1433240.15359088894954576No Hit
CGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGC1409400.15103611320189905No Hit
CCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTC1395180.14951224947993863No Hit
CTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCC1391780.14914789387834473No Hit
CCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTT1387790.14872031186353307No Hit
CCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGT1372350.14706570877864777No Hit
CGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTT1345730.1442130187449919No Hit
CTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAGT1342790.1438979583130254No Hit
CACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAAT1335870.14315638750036958No Hit
CTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTC1329280.1424501806137508No Hit
GTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCC1324410.14192829479617364No Hit
CTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACAC1308230.14019439078623555No Hit
TGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTCCC1296460.13893307742424724No Hit
GCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTT1294930.13876911740352998No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC1285350.13774249191433302No Hit
AGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCAAAGA1282770.13746601031077058No Hit
CTGGTATCTTCGACTGATTTCAGCTCCATCCGCGAGGGACCTCACCTAC1266550.13572781976434314No Hit
CCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGC1265420.13560672510851932No Hit
ACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCCGG1259350.13495624319626195No Hit
CTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTAT1244590.13337451123169541No Hit
GTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCT1241600.13305409262911724No Hit
CTCGGTACTACATGCTTAGTCAGTCTTTACATTCGCTTGCCAGCTGCGG1237440.1326082928342259No Hit
CCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC1228900.13169311729375177No Hit
CGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACT1206980.1293440952975934No Hit
GCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCT1203660.12898831276897815No Hit
CACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGC1202840.12890043877094667No Hit
CAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTAC1189650.12748695336358679No Hit
CGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCC1187670.12727476980736446No Hit
CGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGAT1154330.12370194164349946No Hit
CCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTGTGTCGG1147680.12298930495214667No Hit
CCCGCTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG1137550.12190373958622126No Hit
CGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCATTCGC1136480.12178907473513143No Hit
AACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGG1107200.11865133002493447No Hit
CTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGGCCTTC1101010.11798798850320907No Hit
GTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCG1092440.11706959806036797No Hit
CGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCACGT1084490.1162176489331116No Hit
CCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTG1066210.11425870175748318No Hit
CTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGG1050320.11255587513709282No Hit
CCCCGCTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT1050240.11254730206411412No Hit
CCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTC1047680.11227296372879637No Hit
GCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTCCCT1045600.11205006383135069No Hit
CGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTG1034140.11082197112715475No Hit
CTCTTCACGACGGACGTTAGCACCCGCCGTGTGTCTCCCGTGATAACATT1031020.1104876212809862No Hit
CCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGA1002480.10742917749584203No Hit
CCGAACACCAGTGATGCGTCCACTCCGGTCCTCTCGTACTAGGAGCAGCC999160.1070733949672268No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG995730.10670582446326587No Hit
GTCGACTTAACGCGTTAGCTCCGGAAGCCACGCCTCAAGGGCACAACCTC993790.10649792744353288No Hit
GTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGAC989870.10607784686757755No Hit
CTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTACATATCAGC985540.1056138292926065No Hit
GCTGCTTCTAAGCCAACATCCTGGCTGTCTGGGCCTTCCCACATCGTTTC982400.10527733617819329No Hit
CGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGCATT974690.10445110626987299No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG971230.10408032086354506No Hit
CTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTTAT966200.10354128890001053No Hit
GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG965090.10342233751243135No Hit
CACGCTTAAACCGGGACAACCGTCGCCCGGCCAACATAGCCTTCTCCGTC956950.10255002733685063No Hit
TCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTC955120.10235391829246333No Hit
CCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGT946350.1014140951671755No Hit
CGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCT944480.10121369958629887No Hit
GCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACC942400.10099079968885318No Hit
TCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGT934270.1001195611473948No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)