FastQCFastQC Report
Wed 13 Sep 2023
SRR12649879_pass.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12649879_pass.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21072517
Sequences flagged as poor quality0
Sequence length35-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTC3175501.5069391093622087No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACT3106991.4744275683820778No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG2777501.3180675094484442No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT2674511.2691934238325682No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC2380541.1296894433636No Hit
CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTC2272951.0786324196582686No Hit
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG2098690.9959370302085888No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT1930720.9162265713203601No Hit
CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG1816400.8619758142798034No Hit
CCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGC1793950.8513221272997431No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG1426320.6768626642939711No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC1407450.6679078726096175No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC1273320.6042562452316446No Hit
CTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTAT1187980.563757998154658No Hit
TTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGG1180920.5604076627391024No Hit
CCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGT1144090.5429299214707004No Hit
CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG1069200.5073907402708466No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCC924620.4387800470157409No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGC915960.43467042878646156No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG867360.41160721331960487No Hit
CACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATT861060.40861753724056793No Hit
CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT803450.38127861042892974No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCG780640.3704540848157816No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT762830.36200231799551996No Hit
CCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA755390.35847165291170485No Hit
CTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTT751690.35671581140496883No Hit
CTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTG728180.3455590995608166No Hit
CTCCAATCCGGACTACGACGCACTTTATGAGGTCCGCTTGCTCTCGCG690800.3278203548251972No Hit
CTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTG670220.31805407963367643No Hit
ATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGG649410.30817865753768287No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT643640.30544049389069183No Hit
CGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT631510.2996841810591492No Hit
CTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGC599230.28436565029227406No Hit
CGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT591990.2809298955601744No Hit
CTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGT577710.2741532964476906No Hit
CTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCAT575860.2732753756943226No Hit
CGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCG569260.2701433340877124No Hit
CTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCT537540.25509055230564054No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG520070.24680013308329518No Hit
CACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCC514150.24399078667251758No Hit
CGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC487500.2313439823064326No Hit
CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC481720.22860107314185582No Hit
GTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTCC473110.22451518250050528No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC470430.22324338378751812No Hit
CTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTC460300.2184361744731301No Hit
CACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCC448890.21302153890776312No Hit
GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG433560.20574666044877316No Hit
CCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGG428300.20325051819865655No Hit
GGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGG389410.18479520030758545No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG379740.180206284802143No Hit
ATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGCATTTG376570.17870195572745295No Hit
CTTTGGTTTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTAGTTAT370730.17593057345736154No Hit
CTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGC368720.1749767244226212No Hit
GTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCT360110.17089083378127065No Hit
CACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGT354250.16810996047600768No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACC349030.16563280029623417No Hit
CGCTACACCTGGAATTCTACCCCCCTCTACGAGACTCAAGCTTGCCAGT348410.16533857820591627No Hit
GATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT348170.1652246857838577No Hit
CTTTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGGC344920.1636823925684815No Hit
CCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATAC332620.15784540593798074No Hit
CTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCTCACGACG329730.15647395135569234No Hit
CTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACAC329580.15640276859190577No Hit
CTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC325970.15468963674344172No Hit
GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGC324200.1538496801307599No Hit
CGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTG323290.15341783803045456No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCAT321790.1527060103925886No Hit
CTGGTATCTTCGACTGATTTCAGCTCCACGAGCAAGTCGCTTCACCTAC314120.14906619840430074No Hit
CCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTC311640.14788931004302905No Hit
CAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCAA310140.1471774824051631No Hit
CCGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGT308960.14661751133004186No Hit
ATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC307500.14592466576251903No Hit
CTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCC306060.1452413112301677No Hit
CCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTG299710.14222790756320186No Hit
GTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGC298540.14167268200566643No Hit
CGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTT298460.14163471786498025No Hit
CGGGTTTCGGGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTT298390.14160149924187984No Hit
ACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCCGG296780.1408374709105704No Hit
CGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGCCCGGG293980.13950872598655395No Hit
CACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGC293820.13943279770518158No Hit
CCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTC291780.1384647121176839No Hit
GGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTT289840.13754408170604393No Hit
GGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTC282550.13408459938601544No Hit
CTCGGTACTACATGCTTAGTCAGTCTTTACATTCGCTTGCCAGCTGCGG278620.13221961097480667No Hit
CTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATT277410.13164540334692812No Hit
GCCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCT274970.13048749705599955No Hit
GGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCC273230.12966177699607503No Hit
CCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCTT268900.12760696788143533No Hit
ATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTT268820.12756900374074914No Hit
CCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGC268650.12748832994179102No Hit
TCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTC268240.12729376372077433No Hit
CCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGT263110.12485931319927278No Hit
CATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCA262530.12458407317929795No Hit
CCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGT262390.12451763593309711No Hit
CTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGG261220.12396241037556169No Hit
GCGCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTT253620.12035581701037423No Hit
CTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAGT253230.12017074182452908No Hit
GTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC250610.11892741621705655No Hit
CGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTACGC250240.11875183206638296No Hit
CCCCGCTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT247680.11753697956442506No Hit
CCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAGGT245670.1165831305296847No Hit
GCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTT244090.11583333875113258No Hit
CCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC243240.11542996975634189No Hit
CCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC243080.11535404147496951No Hit
CACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCC242920.11527811319359714No Hit
ATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTC242660.11515472973636703No Hit
GTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCG240940.11433850071161408No Hit
CAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCGC238730.11328974132515826No Hit
GTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGAC236420.1121935267628447No Hit
TCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGT236250.11211285296388657No Hit
CTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTC235740.11187083156701215No Hit
CACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAAT235730.11186608604942638No Hit
GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG231920.11005804384924686No Hit
CTTTCACCCCCAGCCACAAGTCATCCGCTAATTTTTCAACATTAGTCGGT231060.10964992933687039No Hit
GGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGAC225710.10711107742848185No Hit
GTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCT225630.10707311328779566No Hit
ACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTC221470.1050989779721141No Hit
CCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGT218180.1035377026863948No Hit
AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC214340.10171542393345796No Hit
CCGCCTTCGCCACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTAC214040.10157305840588478No Hit
CCGCCGTGTGTCTCCCGTGATAACATTCTCCGGTATTCGCAGTTTGCATC213040.10109850664730749No Hit
CTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACAC210850.10005923829602321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)