FastQCFastQC Report
Tue 7 May 2024
KC14_103_WT_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameKC14_103_WT_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45646517
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC741800.16250966092330768No Hit
CTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGA597500.13089717228589423No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT504370.11049473939052129No Hit
CTCGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCT494860.10841133837221359No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT484220.10608038286908068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)