FastQCFastQC Report
Mon 20 Jun 2022
SRR13762417_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762417_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21872963
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1892650.8652920045628935No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT1649400.7540816486545513No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA1295430.5922517219089156No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGTCACGAGTGTAGATCTC1254780.5736671341692481Illumina Single End PCR Primer 1 (96% over 33bp)
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC996730.45569043389320407No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT922270.42164840675678006No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC887940.4059532309362934No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG800770.3661003769813902No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG746730.34139407633067365No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA622460.28457964291349097No Hit
CTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGT604760.2764874607980638No Hit
AAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAG573000.2619672515333199No Hit
AAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGA555570.25399850948406033No Hit
AGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGA502900.22991855287278637No Hit
CCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTC431690.19736237838467516No Hit
CTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGTCTCCG400450.18307990554366138No Hit
CGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCT332880.1521878860216606No Hit
CGGAGAGCTCCGGGGAGGTCACCCACACCCTTCAGGATGAAAGCTGTGGT325610.14886414794374225No Hit
CACCCAGCTCTGGGACAGCAAGCTGAAAATGACTCCCTCCATCTCATGGG315800.14437915887298855No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT299110.136748734042114No Hit
GACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATG296670.1356332015923037No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC273820.12518651451108842No Hit
CTCAACCAATCACAAAGATATCGGAACCCTCTATCTACTATTCGGAGCCT268740.12286401252541779No Hit
CAACAACCCTGAACATCAGGAGTCAGCTATCACAGAGGCTCTGCAGCCTG267070.1221005128569001No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGA260700.11918824166620681No Hit
GTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTG257290.1176292393490539No Hit
GTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTG239270.10939075789594671No Hit
CTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTAACTGGAGCC224160.10248268604486736No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT223230.10205750359473474No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)