FastQCFastQC Report
Mon 20 Jun 2022
SRR13762415_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762415_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20498294
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1429310.6972824177465695No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT1050730.5125938773246204No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA979120.47765926276596476No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG867900.423401088890617No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCATCCTTGTGTAGATCTC785030.38297333426869573Illumina Single End PCR Primer 1 (96% over 31bp)
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT652670.318402107024126No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC608270.29674176787590223No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC595360.2904436827767228No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG551390.2689931171833129No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA399410.19485036169351458No Hit
CTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGT374120.18251274959760067No Hit
AAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGA357710.1745072053313315No Hit
CCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTC344660.16814082186546842No Hit
AAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAG334360.16311601345946156No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCATCCTTGTGTAGATCT308370.15043690953012967Illumina Single End PCR Primer 1 (96% over 32bp)
AGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGA301170.14692442210068798No Hit
CTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGTCTCCG256280.1250250386690717No Hit
CGGAGAGCTCCGGGGAGGTCACCCACACCCTTCAGGATGAAAGCTGTGGT253050.12344929778058604No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT236930.11558522870244714No Hit
CTCAACCAATCACAAAGATATCGGAACCCTCTATCTACTATTCGGAGCCT215480.10512094323556877No Hit
CACCCAGCTCTGGGACAGCAAGCTGAAAATGACTCCCTCCATCTCATGGG212730.10377936817571258No Hit
GACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATG211730.10329152269940123No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT206030.10051080348442655No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)