FastQCFastQC Report
Mon 20 Jun 2022
SRR13762415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20498294
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG904470.4412415979593229No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGTCTATCTCGTATG730870.3565516232716732TruSeq Adapter, Index 2 (97% over 35bp)
CTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCTGCATGGA666800.3252953636044053No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC513450.25048425981206046No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG427700.20865151021836253No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC317270.15477873426930064No Hit
GGGCGATCTCACTCTTGTGTGCTTCTCGGCGAAACACACCCCTGGAAAAA305310.14894410237261696No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGTCTATCTCGTAT296580.14468521136441892TruSeq Adapter, Index 2 (97% over 36bp)
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA278250.13574300378363197No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC257040.1253958012310683No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA241410.11777077643632197No Hit
CAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCA234410.11435585810214255No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)