FastQCFastQC Report
Mon 20 Jun 2022
SRR13762414_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762414_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18521780
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1772250.9568464801979075No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1042630.5629210583432046No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT888040.4794571580053321No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC801980.43299294128318117No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA783630.4230856861489555No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT523120.28243505753766646No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC520360.280944919980693No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG433230.2339030050027589No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA331010.17871392490354598No Hit
AAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGA297680.16071889418835553No Hit
AAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAG292410.15787359530239534No Hit
CTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGT276490.14927830910420056No Hit
CGGAGAGCTCCGGGGAGGTCACCCACACCCTTCAGGATGAAAGCTGTGGT272790.14728066092999703No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT265270.14322057599215626No Hit
AGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGA264750.14293982543794387No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTTATAGTGTAGATCTC264440.14277245491524032Illumina Single End PCR Primer 1 (97% over 35bp)
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT243720.1315856251397004No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGA243600.13152083655026675No Hit
CCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTC222090.11990748189428878No Hit
CTCAACCAATCACAAAGATATCGGAACCCTCTATCTACTATTCGGAGCCT219250.11837415194435956No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTTATAGTGTAGATCT214620.11587439220204537Illumina Single End PCR Primer 1 (97% over 36bp)
CTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTAACTGGAGCC190650.102932871462678No Hit
CTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGTCTCCG188070.10153991678985497No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)