FastQCFastQC Report
Mon 20 Jun 2022
SRR13762414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18521780
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG1619360.8743004182103448No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG465400.25127174602009095No Hit
CTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCTGCATGGA458350.24746541639086525No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC361950.19541858287918332No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC341460.18435593123339117No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC299900.16191748309287768No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA273970.14791774872609437No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTCGTCATCTCGTATG251710.1358994653861562TruSeq Adapter, Index 1 (97% over 35bp)
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA250950.13548913765307652No Hit
GTTGGTTCCTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTC235260.12701802958462954No Hit
CAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCA213610.115329088240979No Hit
CGGGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCT208460.11254857794445242No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTCGTCATCTCGTAT206560.11152275861175331TruSeq Adapter, Index 1 (97% over 36bp)
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT204020.11015140013540817No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC190170.10267371710494348No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC188880.10197723976853197No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)