FastQCFastQC Report
Mon 20 Jun 2022
SRR13762409_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762409_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22234225
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2837851.2763431151749163No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC1445270.6500204077272763No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1116820.5022976964567013No Hit
CTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGT538150.24203676988966333No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGCTATGTGTGTAGATCTC394040.177222277817194Illumina Single End PCR Primer 1 (96% over 33bp)
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGA383330.1724053795443736No Hit
CCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTC364280.16383750726638774No Hit
CTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGTCTCCG360510.16214192309378897No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT323950.1456988044332555No Hit
CTCAACCAATCACAAAGATATCGGAACCCTCTATCTACTATTCGGAGCCT311070.13990593330777215No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT291280.13100524079431597No Hit
CTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTAACTGGAGCC261110.11743606984277617No Hit
CAAGACGCCACATCCCCTATTATAGAAGAGCTAATAAATTTCCATGATCA259160.11655904354660439No Hit
CTACAAGACGCCACATCCCCTATTATAGAAGAGCTAATAAATTTCCATGA249310.1121289363582495No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)