FastQCFastQC Report
Mon 20 Jun 2022
SRR13762409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22234225
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG964150.4336332838225754No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC650690.2926524311056491No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA486370.21874834854824038No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG476890.21448465147762066No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC371920.16727365131908128No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGCTAGATCTCGTATG355850.1600460551244759TruSeq Adapter, Index 2 (97% over 35bp)
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT298420.13421650630952958No Hit
CCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAAC282660.12712833480816174No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA252340.11349170029537796No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC223320.10043974998004201No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)