FastQCFastQC Report
Mon 20 Jun 2022
SRR13762408_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762408_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23858868
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT3169741.3285374645603472No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC1655920.6940480160249011No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1275480.5345936781242094No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT1004430.42098812064344376No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTATGTTCGTGTAGATCTC900350.3773649277912095Illumina Single End PCR Primer 1 (96% over 31bp)
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA849800.3561778371044259No Hit
CTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGT730380.30612516905663756No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC618830.2593710648803623No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT536310.224784344336873No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGA473280.19836649416896057No Hit
CTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGTCTCCG469610.19682828204590427No Hit
CCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTC436500.18295084242890317No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG430830.1805743675684865No Hit
AAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAG365460.15317574999786243No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA363040.15216145208565637No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT358760.15036756982770516No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT345220.14469253109577537No Hit
AAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGA341020.14293217934731858No Hit
CTCAACCAATCACAAAGATATCGGAACCCTCTATCTACTATTCGGAGCCT325350.1363643908001No Hit
AGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGA316600.1326969913241483No Hit
CACCCAGCTCTGGGACAGCAAGCTGAAAATGACTCCCTCCATCTCATGGG287890.12066372972934006No Hit
CTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTAACTGGAGCC255000.10687849901344858No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTATGTTCGTGTAGATCT242430.10161001770913859Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)