FastQCFastQC Report
Mon 20 Jun 2022
SRR13762408_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762408_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23858868
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG2108390.883692386411627No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTGTCAATCTCGTATG850190.3563412983382112TruSeq Adapter, Index 8 (97% over 37bp)
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC689130.2888360000985797No Hit
CTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCTGCATGGA584380.24493198922932977No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG560950.2351117412611529No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA481500.2018117540195117No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT395430.16573711711720773No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC376270.15770656009329528No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC353720.148255147729557No Hit
CCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAAC314980.1320179985068864No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA309700.12980498488025502No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC299950.12571845403562315No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC274070.11487133421417982No Hit
GGGCGATCTCACTCTTGTGTGCTTCTCGGCGAAACACACCCCTGGAAAAA247590.10377273557152836No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA243170.10192017492196193No Hit
CAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCA240100.10063344162011374No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)