FastQCFastQC Report
Mon 20 Jun 2022
SRR13762407_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762407_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19603570
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2516731.2838120811668488No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC1174780.5992683985620986No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1074700.5482164728159208No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT701030.3576032324724527No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA529830.2702722004206377No Hit
CTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGT436730.22278085063077796No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC426270.21744508780798597No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT397990.20301914396204365No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGA354840.18100784704010547No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT327340.16697978990561413No Hit
CCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTC318740.1625928338562823No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG302330.15422190958075493No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGCACCTGTGTAGATCTC291440.14866679895549637Illumina Single End PCR Primer 1 (96% over 32bp)
CTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGTCTCCG281810.14375442840258176No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA275550.14056113248760302No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT268790.13711278098836077No Hit
CACCCAGCTCTGGGACAGCAAGCTGAAAATGACTCCCTCCATCTCATGGG242470.1236866550327313No Hit
AAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAG215210.10978102457868642No Hit
CTCAACCAATCACAAAGATATCGGAACCCTCTATCTACTATTCGGAGCCT204940.1045421828779146No Hit
AGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGA200920.10249153598043623No Hit
AAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGA198300.1011550447188956No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)