FastQCFastQC Report
Mon 20 Jun 2022
SRR13762406_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13762406_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20297329
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2194061.0809599627616029No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1460040.719326173409319No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC1069250.5267934514930511No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTACTCATAGTGTAGATCTC388560.1914340551902174Illumina Single End PCR Primer 1 (96% over 31bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT376050.18527068265977262No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT349220.17205219465083313No Hit
CTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGT349200.172042341137595No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGA330140.16265194302166555No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTACTCATAGTGTAGATCT280020.137959038846934Illumina Single End PCR Primer 1 (96% over 32bp)
CCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTC278080.13700324806283623No Hit
CGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAACGGAACAGACCCGGGG241190.11882844289512183No Hit
CTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTCTTCGTCTCCG238650.11757704671388042No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)