FastQCFastQC Report
Tue 12 Jul 2022
SRR4066388_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4066388_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31025536
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGA2511920.8096298481354198No Hit
GGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGAT560890.18078333924674178No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC541330.17447885509536404No Hit
CCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTC495630.1597490531670428No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAG457170.14735281285712518No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG453790.1462633876816826No Hit
GCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG449510.14488387887964288No Hit
CTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTA445070.14345279965509702No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG413300.13321284763621813No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTT410590.13233937360502007No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGA405630.13074069050732917No Hit
GTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATC381310.12290198628639325No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC374290.12063933399893559No Hit
CAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA371540.119752967362111No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTC355930.11472162801635401No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCAC354150.11414790706597301No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC340740.10982566102967568No Hit
CGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAG322260.1038692772302145No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)