FastQCFastQC Report
Wed 27 Sep 2023
SRR16082813_pass_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16082813_pass_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16561808
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT799610.482803568306069No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG500340.3021046977479753No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT467420.28222764084694135No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC322090.19447755945486145No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC194220.11726980532560213No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)