FastQCFastQC Report
Wed 27 Sep 2023
SRR16082810_pass_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16082810_pass_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16931511
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT784840.4635380740679317No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG429560.25370446854979456No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT387820.22905220922101993No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC320430.189250681761362No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC287880.1700261719110598No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA278130.1642676781770983No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT217440.12842326948847033No Hit
CCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCC198890.1174673660253949No Hit
AGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTC186810.11033273994270211No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT170910.10094196554578029No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)