FastQCFastQC Report
Wed 27 Sep 2023
SRR16082806_pass_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16082806_pass_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14555720
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGC606760.4168533057794461No Hit
GGCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT343650.23609275253989498No Hit
GGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT319060.21919905027027176No Hit
GGGGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCA298860.20532134446114655No Hit
GGGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCC275940.1895749574737629No Hit
GGGGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGG268550.1844979155960681No Hit
GGGGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAG224510.15424176887161886No Hit
CGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT206650.14197167848790715No Hit
GGGGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG190890.13114431989623324No Hit
CGGGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGC188630.12959166568194497No Hit
GCCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT177830.12217190218003644No Hit
CGCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT169750.11662081985638637No Hit
GGGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCAC157090.1079232081958158No Hit
GGGGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGC150010.10305914101123133No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)