FastQCFastQC Report
Wed 27 Sep 2023
SRR16082805_pass_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16082805_pass_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12859634
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT442570.34415442927846934No Hit
GGGGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGC437320.3400718869603909No Hit
GGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT409640.31854716860526516No Hit
CGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT261240.20314730574758194No Hit
GGGGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCA260660.20269628202482282No Hit
GCCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT232730.18097715689264562No Hit
CGCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT218770.17012148246209807No Hit
GGGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCC207290.16119432325989994No Hit
GGGGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGG175410.13640357105031137No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161740.1257734084811434No Hit
GGGGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAG143810.11183055443102034No Hit
CGGGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGC135730.10554732739672062No Hit
GGGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCAC134260.10440421554765866No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)