FastQCFastQC Report
Wed 27 Sep 2023
SRR16082805_pass_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16082805_pass_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12859634
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG1225140.9527020753467789No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA1146890.8918527541297054No Hit
AGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC487130.37880549322010254No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT359120.2792614470987277No Hit
TTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTA344550.2679314201321748No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATT320070.24889510852330632No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA312740.2431951018201607No Hit
TTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCA305070.23723070190022516No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA283190.22021622077269074No Hit
TGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA192960.1500509267993164No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC191690.1490633403718955No Hit
TTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCC190770.1483479234323465No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA190300.14798243869148997No Hit
TCCTTTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG167190.1300114762208629No Hit
TTCCCTTGCGGTACTAGTTCTATAGCTCCTAGATGTACGAATTTCTTTCT159800.1242648118912249No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACATCGCCGCC149790.11648076453808871No Hit
GGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCAT142720.11098294088307645No Hit
AGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAA141050.10968430361237341No Hit
TCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCT136280.1059750223062336No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)