FastQCFastQC Report
Wed 27 Sep 2023
SRR16082804_pass_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16082804_pass_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19817731
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA3164221.5966610910199557No Hit
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG2128581.0740785612641528No Hit
AGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC911350.45986596548313224No Hit
TTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCA641750.3238261736421793No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA565140.28516887225888776No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC543770.27438559944122765No Hit
TGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA538490.2717213186514642No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA487520.24600192625482703No Hit
TTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTA431950.21796138013983538No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT423820.21385899324196095No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATT399080.2013752230262889No Hit
TCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCT380000.19174748108146186No Hit
TTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCC355160.17921325100234733No Hit
TCCTTTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG307890.15536087355308234No Hit
GCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCAC306480.15464938947854323No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA249990.12614461261988064No Hit
CCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGT229740.11592649027277645No Hit
TTCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG224860.11346404893678291No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC222520.11228328813222867No Hit
AGTTAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC219560.11078967617433097No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACATCGCCGCC217100.1095483635336457No Hit
AGATGGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC212820.10738868137830714No Hit
ACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGG212680.10731803756948764No Hit
GTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGG209750.1058395635706227No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA207260.1045831129709047No Hit
CTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGG204910.10339730618000617No Hit
AGATTGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC201380.10161607300048629No Hit
GATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCG201370.10161102701414203No Hit
TTTGGGTCCTAACGCGTACGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC200090.10096514076207816No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)