FastQCFastQC Report
Wed 27 Sep 2023
SRR16082803_pass_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16082803_pass_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9993591
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA1410671.4115746782112657No Hit
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG1066281.0669638171103861No Hit
AGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC481430.48173874636254377No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT314920.3151219616652312No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA314210.31441150633440973No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATT302750.30294415691016374No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA299440.2996320341707No Hit
TTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTA285530.2857131135344642No Hit
TTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCA285400.28558303016403214No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC237270.23742216386482096No Hit
TGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA234970.23512068884948364No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA195620.19574545326099496No Hit
TTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCC189380.18950145148025369No Hit
TCCTTTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG171100.17120972831487702No Hit
TCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCT168460.16856803525379416No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACATCGCCGCC138560.13864886005440888No Hit
AGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAA133710.13379574969598015No Hit
GGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCAT131240.13132416565777005No Hit
GCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCAC127650.12773186335122177No Hit
TTTGGGTCCTAACGCGTACGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC125970.12605078594871452No Hit
CCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGT125750.12583064486029097No Hit
AGTTAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC121790.1218681052686667No Hit
TTCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG119710.11978677134175293No Hit
AGATGGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC118930.11900627111916028No Hit
TCCCATTTCATTGGCTACACCTTGACCTAACGTTTTTATGTTTGATTCTT118660.11873609796518589No Hit
CTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGG117240.11731518730354283No Hit
ACGAATGGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGG114680.1147535455473413No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA114440.11451339163269739No Hit
AGATTGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC111970.1120418075944873No Hit
GATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCG108110.10817933213396466No Hit
TTCCCTTGCGGTACTAGTTCTATAGCTCCTAGATGTACGAATTTCTTTCT107050.10711865234428745No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA105430.10549761342044117No Hit
TGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC100750.10081461208488521No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)