FastQCFastQC Report
Wed 27 Sep 2023
SRR16082801_pass_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16082801_pass_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18089361
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA2533021.4002816351556033No Hit
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG2292411.267269750435076No Hit
AGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC997470.5514125125812902No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA778280.43024184215241207No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA656820.363097402943089No Hit
TTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCA572210.3163240536799503No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT444890.2459401412797279No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC425300.23511057134632893No Hit
TGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA421250.2328716862911852No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATT373850.20666843897913256No Hit
TTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCC350800.19392614255417867No Hit
TCCTTTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG329820.18232816515740938No Hit
TTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTA324330.179293232082659No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACATCGCCGCC313810.1734776590505325No Hit
TCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCT312000.17247707091477693No Hit
AGATGGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC252550.13961244954976573No Hit
AGTTAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC247140.1366217413649935No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA244990.13543319744683074No Hit
CCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGT242660.1341451475262172No Hit
ACGAATGGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGG232660.12861703627894872No Hit
AGATTGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC232110.12831299016034894No Hit
TTCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG226940.12545495664551112No Hit
CTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGG226670.12530569764183488No Hit
GATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCG225250.12452070584472276No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC224990.12437697495229379No Hit
TTTGGGTCCTAACGCGTACGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC213830.11820760280034215No Hit
TGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC208370.11518925405933356No Hit
GCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCAC204570.11308857178537152No Hit
ACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGG187870.10385662600243314No Hit
ACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGG186280.10297765631411746No Hit
GCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCC185980.1028118129766994No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)