FastQCFastQC Report
Wed 27 Sep 2023
SRR16082800_pass_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16082800_pass_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16936993
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGC631300.3727344045073408No Hit
GGCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT350050.2066777733213918No Hit
GGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT332390.1962508929418581No Hit
GGGGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCA316800.18704618936785294No Hit
GGGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCC280820.16580274904760248No Hit
GGGGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGG279600.16508243228299144No Hit
GGGGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAG226250.13358333442069675No Hit
CGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT211670.12497495866001715No Hit
GGGGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG205210.12116082234904389No Hit
GGGGGGCCCCGCGGACGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGT191450.11303659392195534No Hit
CGGGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGC191330.11296574309264934No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG182770.10791171726882097No Hit
GCCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT177940.10505997138925428No Hit
CGCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT175170.10342449807944068No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG174790.10320013711997164No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)