FastQCFastQC Report
Wed 27 Sep 2023
SRR16082799_pass_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16082799_pass_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15684837
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGC676020.4310022475847215No Hit
GGCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT425650.27137674430406894No Hit
GGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT405560.25856819551264704No Hit
GGGGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCA345700.2204039480933082No Hit
GGGGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGG309890.1975729808349299No Hit
GGGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCC303270.19335234405049923No Hit
CGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT263310.16787550932151862No Hit
GGGGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAG253310.16149992505500693No Hit
GCCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT225380.14369291819863989No Hit
CGCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT216940.13831192507770404No Hit
GGGGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG213800.13630999161801935No Hit
CGGGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGC205730.13116489511494445No Hit
GGGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCAC177380.11309011371938389No Hit
GGGGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGC164320.10476360066731966No Hit
GGGGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAGAAGAC158200.10086174309621451No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)