FastQCFastQC Report
Wed 27 Sep 2023
SRR16082799_pass_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16082799_pass_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15684837
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA2684881.7117678685471835No Hit
TCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG1675051.0679422425620362No Hit
AGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC754090.48077643395337805No Hit
TTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCA506150.3227001976494879No Hit
TGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA479160.30549249571417286No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC468170.29848572860527656No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT434600.2770828922225969No Hit
CGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAA421980.2690369048782592No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA398070.2537928828970298No Hit
TTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTA386120.24617405969854836No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATT349120.22258439791245518No Hit
TCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCT333880.21286800749029142No Hit
TTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCC314290.20037823791219508No Hit
TCCTTTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG282540.18013575786602054No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA246030.15685849970898644No Hit
GCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCAC230730.14710385578122362No Hit
CCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGT202540.12913108373392723No Hit
TTCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCG199350.12709727235291002No Hit
AGTTAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC196190.12508258772469233No Hit
TCCCATTTCATTGGCTACACCTTGACCTAACGTTTTTATGTTTGATTCTT190290.12132099300745045No Hit
CTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGG188810.1203774065360067No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC188060.11989923771601833No Hit
GTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGG177910.113428019685509No Hit
TTTGGGTCCTAACGCGTACGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC177720.11330688358444528No Hit
AGATGGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC177260.11301360670818575No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACATCGCCGCC176570.11257369139379644No Hit
GGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCAT176230.11235692152873505No Hit
AGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAA174710.11138783272022527No Hit
AGATTGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC171860.10957079120426944No Hit
ACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGG170280.1085634488901606No Hit
GATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCG170030.1084040592834978No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA169220.10788763695791037No Hit
TGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCC163130.10400490613960477No Hit
ACGAATGGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGG162090.10334184537588754No Hit
ACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGG161010.1026532822751043No Hit
TTTGCTTACTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATA159490.10168419346659452No Hit
TCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTG157410.1003580719391601No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)