FastQCFastQC Report
Tue 19 Jul 2022
GSM4964638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGSM4964638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48301199
Sequences flagged as poor quality0
Sequence length35-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTC3808000.7883862261887121No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCC3432090.7105600007983238No Hit
CTGCGTACGTCGGGAGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGG1984640.4108883508254112No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC1723510.35682551068763324No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTG1487200.30790125934554957No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGC1355030.28053754938878434No Hit
GTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCG1314860.2722209856529649No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG1259100.26067675876948726No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG1186130.2455694733375045No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCCGGGAAGGGGGGTC1167320.24167516007211334No Hit
CTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCC1091290.2259343499940861No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT926540.19182546586472937No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCC915600.18956051173802124No Hit
GTGGGTCTTCCGTACGCCACATTTCCCACGCCGCGACGCGCGGCGGGGATT906820.18774275147910924No Hit
GGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCC814930.16871837901994938No Hit
GTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCG771510.15972895414045518No Hit
CCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTC698410.14459475426272544No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC693830.14364653763563923No Hit
CCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT692890.1434519254894687No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTG673780.13949550196466137No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTC653540.1353051297960533No Hit
GTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATC631840.13081248769828677No Hit
GTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCC630700.13057646871250547No Hit
CTCCCGTCCCGTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCG615180.12736329795871112No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGG586450.12141520544862665No Hit
GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACT584520.1210156294463829No Hit
GGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA573940.11882520763097414No Hit
GGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATT569690.11794531228924567No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC568610.1177217153553476No Hit
GTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT566740.11733456140498708No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACG558960.11572383534412883No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCATT542190.11225187184276729No Hit
CCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGG538110.11140717231470797No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGC537410.11126224837607034No Hit
CCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCT535200.11080470279837151No Hit
CCCGTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCC530530.10983785309346047No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACT528480.10941343298745028No Hit
CTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCG524160.10851904525185803No Hit
CCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGGG506230.10480692208075415No Hit
GGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATT498040.10311131199869385No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGT493930.10226040144469292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)