FastQCFastQC Report
Tue 19 Jul 2022
GSM4964637_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGSM4964637_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32542629
Sequences flagged as poor quality0
Sequence length35-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTC2729050.8386077228118233No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCC1963690.6034208238062143No Hit
CTGCGTACGTCGGGAGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGG1803140.5540855350070211No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC1131610.34773158616041744No Hit
GTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCG962740.29583965081616487No Hit
GTGGGTCTTCCGTACGCCACATTTCCCACGCCGCGACGCGCGGCGGGGATT820560.2521492655064838No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG776020.23846260239146627No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC766730.23560788527564874No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGC717360.22043701509180466No Hit
GGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATT715620.21990233179992927No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCC672870.20676571643919733No Hit
CTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCC648730.1993477539875466No Hit
GGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATT635540.1952946088037325No Hit
GGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCC629510.19344165463706084No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTC629180.1933402491851534No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTG623090.1914688576635895No Hit
GTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCG608680.18704081959696617No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCCGGGAAGGGGGGTC589550.18116237627881876No Hit
ATCGCCAGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTC579850.1781816705712375No Hit
GGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA551810.1695652800515902No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT518550.15934483965631663No Hit
GTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT482400.1482363333337328No Hit
GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACT482400.1482363333337328No Hit
CCCGTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCC482150.14815951102168176No Hit
CCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTC471610.1449206823456089No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAAT468560.1439834501385859No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG460100.14138378309877792No Hit
GGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCAT433870.1333235861183803No Hit
GGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG426000.1309052197350128No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN421390.1294886163007912No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTG407110.1251005258364344No Hit
CCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT403020.12384371281127901No Hit
CTCCCGTCCCGTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCG400630.12310929150807084No Hit
CGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGC395560.12155133501967527No Hit
GTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATC382570.117559647685502No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACG347380.10674613904119423No Hit
GTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCC345530.10617765393201638No Hit
CCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGG339360.10428167927059612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)