FastQCFastQC Report
Tue 19 Jul 2022
GSM4964636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGSM4964636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44264010
Sequences flagged as poor quality0
Sequence length35-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTC4229880.9556025312663718No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCC2822790.6377167364637772No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGC1753860.3962270928458583No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG1646920.3720675103769405No Hit
CTGCGTACGTCGGGAGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGG1472930.33276018146570996No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC1458040.32939627476136935No Hit
GGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCC1265480.28589366394956084No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCCGGGAAGGGGGGTC1263300.28540116451265934No Hit
GTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCG1197100.27044544766730355No Hit
GTGGGTCTTCCGTACGCCACATTTCCCACGCCGCGACGCGCGGCGGGGATT1172620.26491499527494233No Hit
CTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCC1156100.26118284357879007No Hit
GGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATT1147610.25926480678094915No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTG1082690.24459826391689318No Hit
GGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATT1051690.23759483155728547No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCC992900.2243131609630488No Hit
GGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA957100.2162253261735663No Hit
ATCGCCAGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTC944260.21332454967365136No Hit
GGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCAT805850.1820553537738673No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC804620.1817774756512119No Hit
GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACT770430.17405336751008324No Hit
GTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCC752010.16989197318543892No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAAT705730.15943652642406325No Hit
CCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCC678690.1533277260691022No Hit
GTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCG648140.14642595643729522No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTG613850.13867925657887753No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTC609320.13765585178568324No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG609260.13764229675530978No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGC568770.12849491042497052No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT560050.12652491267736476No Hit
CCCCCACTACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCG558850.12625381206989608No Hit
CCCGTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCC546370.12343436575222172No Hit
GTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT545890.12332592550923424No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCG538520.12166091594503073No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGG511840.11563344577231029No Hit
CCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTC491820.11111058397104104No Hit
GTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATC485190.10961275311477654No Hit
CGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGC475100.10733324884031067No Hit
GGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTAT473450.10696048550504123No Hit
CGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAAT453420.10243536453204308No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACG447610.10112278575754885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)