FastQCFastQC Report
Tue 19 Jul 2022
GSM4964630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGSM4964630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38494311
Sequences flagged as poor quality0
Sequence length35-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTC4212221.094244809317408No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCC2259750.5870347958689273No Hit
GTGGGTCTTCCGTACGCCACATTTCCCACGCCGCGACGCGCGGCGGGGATT1680140.43646449471455667No Hit
CTGCGTACGTCGGGAGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGG1649350.42846591019644437No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTG1579260.4102580248806116No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGC1575770.4093513974051906No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC1332300.3461030904021116No Hit
GTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCG1253590.3256559131555829No Hit
GTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCG1200240.3117967223780158No Hit
CCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTC1026380.2666316069405684No Hit
GTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATC887780.23062628656998174No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCCGGGAAGGGGGGTC882240.2291871128697433No Hit
CTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCC866390.22506962132664227No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC855270.22218088278031525No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTC828140.21513308810748683No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGT819260.2128262537287653No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACG813760.2113974711743769No Hit
GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACT803440.20871655554505184No Hit
GGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCC801730.20827233405996018No Hit
ATCGCCAGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTC764800.19867870865385795No Hit
CGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCC760030.1974395645112339No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG759770.19737202206320825No Hit
CTCCCGTCCCGTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCG739090.191999799658708No Hit
CACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCA693970.1802785871397984No Hit
CCCGTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCC691400.17961095601892965No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG642890.1670090938892243No Hit
CTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCG639690.16617780222121653No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC609540.1583454760367058No Hit
GTCCCGTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTC604430.15701800715435588No Hit
GTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCC593500.15417862655081682No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT589180.15305638279900632No Hit
GTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT586330.1523160136571869No Hit
GGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTAT563800.14646320075711966No Hit
GTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCC513920.1335054418820485No Hit
CCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGG500050.1299023120585273No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCC493130.12810464382646047No Hit
CGTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC482970.1254652927805358No Hit
GGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATT482350.1253042300198593No Hit
GTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATT480500.1248236395242923No Hit
GGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA476720.12384167624145812No Hit
CTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCC476320.12373776478295714No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTT457340.11880716607708602No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTT453340.11776805149207634No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA452490.11754723964276176No Hit
CCGTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCC450590.11705366021488214No Hit
CCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT450530.11703807349610701No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC447260.11618859732286156No Hit
GGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATT433310.11256468520764018No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCATT431590.11211786593608597No Hit
GTCTTCCGTACGCCACATTTCCCACGCCGCGACGCGCGGCGGGGATTCGGC424650.11031500213109413No Hit
CCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACGGG419750.10904208676445722No Hit
GGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTT409510.10638195342683235No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA406010.10547272816494883No Hit
CCCCTCCTTCTCTCCTCGACCCCCCTCTCACGGGCTTCTCAGACACAAACG403100.10471677230435426No Hit
CCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCC401540.10431151761620047No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCG385830.10023039508357481No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)