FastQCFastQC Report
Tue 19 Jul 2022
GSM4964628_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGSM4964628_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35275498
Sequences flagged as poor quality0
Sequence length35-51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA698070.19789089866286227No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN566640.16063274287438833No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAAT546850.15502261654817745No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTT503960.14286403554104327No Hit
GAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA459620.13029440434831No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC421420.11946535807942386No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC416530.11807912676385177No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTA403940.11451007722130528No Hit
GAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG389300.11035988776118767No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG374150.10606512202889383No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT363070.1029241316451436No Hit
CAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAA358460.10161727553782515No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)