FastQCFastQC Report
Tue 19 Jul 2022
GSM4964628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGSM4964628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35275498
Sequences flagged as poor quality0
Sequence length35-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCCAGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTC2004090.5681252182463873No Hit
GTGGGTCTTCCGTACGCCACATTTCCCACGCCGCGACGCGCGGCGGGGATT1616820.45834080074503836No Hit
CTGCGTACGTCGGGAGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGG1092210.309622843595291No Hit
CCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTC1091700.30947826732311473No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTC906670.25702542881180584No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG799330.22659637576200908No Hit
CATCGCCAGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGT774600.21958584397589512No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC751310.21298352754651403No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGC714180.20245780796631133No Hit
GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACT695790.1972445576813685No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCC687740.19496252044407708No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTG637590.18074585368008128No Hit
CTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCAC604410.17133989150202783No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN570020.16159091503116413No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTC521710.14789585677854922No Hit
GGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATT505220.14322122397818451No Hit
CTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCCTTCATGTTATCTCTT491620.13936585672015175No Hit
GTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCG438440.12429023680969721No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC402370.11406500908931179No Hit
GTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCG397830.11277799678405674No Hit
GGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATT387380.10981560061887716No Hit
CATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCG373010.10574195153814697No Hit
CCCGTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCC364370.10329265939774968No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCC356710.10112118048624007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)